CAJ | 학술논문

目的：探讨Smoothened （ SMO）、STAT3和MMP-9蛋白在三阴性乳腺癌组织中的表达及临床意义。方法：应用免疫组织化学SP法检测33例三阴性乳腺癌、30例乳腺增生及18例癌旁正常乳腺组织中SMO、STAT3、MMP-9蛋白的表达情况，并分析它们与三阴性乳腺癌临床病理因素及预后的关系。结果：在三阴性乳腺癌和乳腺增生组织中SMO蛋白的阳性表达率分别为90．9％和60．0％，STAT3蛋白的阳性表达率分别为96．9％和73．3％，MMP-9蛋白的阳性表达率分别为90．9％和36．7％，三者在正常乳腺组织中均无表达；SMO、STAT3和MMP-9蛋白表达在三阴性乳腺癌、乳腺增生及正常乳腺组织中均有显著差异（P＜0．05）。 SMO和STAT3的表达与乳腺癌组织学分级、pTNM分期有关（P＜0．05），其表达均随着组织学分级和临床分期的增高而增高；MMP-9的表达与淋巴结转移有关（P＜0．01）。 SMO和STAT3（r＝0．361，P＜0．05）、SMO和MMP-9（r＝0．633，P＜0．01）、MMP-9和STAT3（r＝0．803，P＜0．01）在三阴性乳腺癌中的表达均呈正相关。 SMO的表达及pTNM分期与三阴性乳腺癌患者的生存时间有关（P＜0．05）。结论：SMO、STAT3和MMP-9在三阴性乳腺癌发生发展过程中可能具有重要作用，可作为三阴性乳腺癌患者治疗的重要靶标。
목적：탐토Smoothened （ SMO）、STAT3화MMP-9단백재삼음성유선암조직중적표체급림상의의。방법：응용면역조직화학SP법검측33례삼음성유선암、30례유선증생급18례암방정상유선조직중SMO、STAT3、MMP-9단백적표체정황，병분석타문여삼음성유선암림상병리인소급예후적관계。결과：재삼음성유선암화유선증생조직중SMO단백적양성표체솔분별위90．9％화60．0％，STAT3단백적양성표체솔분별위96．9％화73．3％，MMP-9단백적양성표체솔분별위90．9％화36．7％，삼자재정상유선조직중균무표체；SMO、STAT3화MMP-9단백표체재삼음성유선암、유선증생급정상유선조직중균유현저차이（P＜0．05）。 SMO화STAT3적표체여유선암조직학분급、pTNM분기유관（P＜0．05），기표체균수착조직학분급화림상분기적증고이증고；MMP-9적표체여림파결전이유관（P＜0．01）。 SMO화STAT3（r＝0．361，P＜0．05）、SMO화MMP-9（r＝0．633，P＜0．01）、MMP-9화STAT3（r＝0．803，P＜0．01）재삼음성유선암중적표체균정정상관。 SMO적표체급pTNM분기여삼음성유선암환자적생존시간유관（P＜0．05）。결론：SMO、STAT3화MMP-9재삼음성유선암발생발전과정중가능구유중요작용，가작위삼음성유선암환자치료적중요파표。
Objective:To investigate the expressions and clinical significances of SMO , STAT3 and MMP-9 in triple negative breast cancer ( TNBC) .Methods:The expressions of SMO , STAT3 and MMP-9 were immunohistochemically detected in 33 cases of TNBC, 30 cases of mammary hyperplasia and 18 cases of normal breast tissue , and the relationships among SMO , STAT3 and MMP-9 and clinicopathological parameters and prognosis of TNBC patients were analyzed .Results:In TNBC and mammary hyperplasia tissue , the positive expression rates of SMO were 90.9%and 60.0%,the positive expression rates of STAT3 were 96.9% and 73.3%, the positive expression rates of MMP-9 were 90.9% and 36.7%, respectively, however, expressions of them were completely absent in normal breast tissue .The significant correlations were observed between expressions of SMO , STAT3 and MMP-9 in TNBC, mammary hyperplasia and normal breast tissue (P<0.05).The expression of SMO and STAT3 was correlated with superior histologic grade and tumor stage .The expression of MMP-9 was correlated with metastasis of lymph node .The positive correlation was manifested between SMO and STAT3(r=0.361,P<0.05),between SMO and MMP-9 (r=0.633,P<0.01),between MMP-9 and STAT3 (r=0.803,P<0.01) in TNBC.The survival time of TNBC patients was correlated with SMO expression and pTNM (P<0.05).Conclusion:SMO, STAT3 and MMP-9 may play important roles in development of TNBC and may be an important therapeutic target in TNBC .