CAJ | 학술논문

目的：研究食蟹猴感染乙型肝炎病毒（ HBV）后的血清学反应。方法：实验室内人工繁育的1～3日龄或成年食蟹猴观察1个月经病毒学筛选后证实为健康动物后各随机分为对照组、感染组，感染组接种HBV携带者血清0．5 ml（ HBV-DNA≥108拷贝）单只笼养，各组从接种后1～12周每日观察行为变化，每1周取血样检测HBV-M、HBV-DNA、肝功能及对于HBsAg阳性食蟹猴在B超引导下取肝组织常规HE染色检测肝组织炎症程度。结果：成年猴接种后未引发HBsAg阳性反应，有3只幼年猴出现HBsAg、HBcAb及2只出现HBV-DNA反应，ALT在攻毒出现HBsAg阳性后1周开始升高，1个月后达到高峰，其值为180 U/L，以后渐降，持续1个月后接近正常。 AST一周后高于正常参考值呈低平曲线，高峰较ALT迟后1个月， HBsAg阳性食蟹猴HE染色可见部分肝组织呈轻微肝炎病变。结论：攻毒后HBV-M、HBV-DNA、ALT、AST及肝组织病理改变提示HBV感染后能产生应答及肝细胞炎症反应。
목적：연구식해후감염을형간염병독（ HBV）후적혈청학반응。방법：실험실내인공번육적1～3일령혹성년식해후관찰1개월경병독학사선후증실위건강동물후각수궤분위대조조、감염조，감염조접충HBV휴대자혈청0．5 ml（ HBV-DNA≥108고패）단지롱양，각조종접충후1～12주매일관찰행위변화，매1주취혈양검측HBV-M、HBV-DNA、간공능급대우HBsAg양성식해후재B초인도하취간조직상규HE염색검측간조직염증정도。결과：성년후접충후미인발HBsAg양성반응，유3지유년후출현HBsAg、HBcAb급2지출현HBV-DNA반응，ALT재공독출현HBsAg양성후1주개시승고，1개월후체도고봉，기치위180 U/L，이후점강，지속1개월후접근정상。 AST일주후고우정상삼고치정저평곡선，고봉교ALT지후1개월， HBsAg양성식해후HE염색가견부분간조직정경미간염병변。결론：공독후HBV-M、HBV-DNA、ALT、AST급간조직병리개변제시HBV감염후능산생응답급간세포염증반응。
Objective:To study the serological reactions in cynomolgus monkeys infected with hepatitis B virus ( HBV). Methods:To select 1 to 3 days old or adult healthy cynomolgus monkeys by artificial breeding to observe the virology screening in laboratory a month to confirmed healthy animals ,randomly divided into control group and infection group .Infection group vaccination serum HBV carriers 0.5 ml (HBV-DNA≥108 copies) single cages,observe each group behavioral changes daily after inoculation 1 to 12 weeks, each week to confirmed the degree of liver inflammation through the HBV-M, HBV-DNA, liver function and on the B-guided, liver tissue inflammation by routine HE staining .Results: Adult monkeys did not induce positive reaction after vaccination , there were three young monkeys appear HBsAg , HBcAb and 2 appear HBV-DNA reaction, ALT poison attack occurred in HBsAg-positive began to increase after one week , one month after the peak , which was 180 U/L, after gradually decreased , continuing a month later near normal .AST higher than a week after the normal reference values were flat curve , representing the peak ALT after a month later, HBsAg positive cynomolgus monkeys HE staining showed mild hepatitis partial liver tissue lesions .Conclusion:HBV-M, HBV-DNA, ALT, AST and liver histopathology after HBV infection have changed , this result showing that it's produce inflammation and induction the response of immune .