CAJ | 학술논문

目的：应用来源于BALB/c小鼠的4T1乳腺癌细胞株建立BALB/c小鼠乳腺癌模型，选择最佳的模型制作方法。方法：90只BALB/c小鼠随机分为3组，每组30只，分别接种1×106 ml-1、1×107 ml-1、1×108 ml-14T1乳腺癌细胞株细胞悬液。每组小鼠再随机分两组分别接种于胸壁和侧腹壁。比较各组的成瘤时间、成瘤率和8周存活率，并用C-erbB-2免疫组化法检查肿瘤病理状态。结果：细胞浓度为1×107 ml-1时，肿瘤成瘤率高，生长稳定；4 T1细胞不同部位接种不影响成瘤率及生长；C-erbB-2免疫组织化学检查均为阳性。结论：应用1×107 ml-1浓度的4T1细胞进行接种是最佳的模型制作方法。
목적：응용래원우BALB/c소서적4T1유선암세포주건립BALB/c소서유선암모형，선택최가적모형제작방법。방법：90지BALB/c소서수궤분위3조，매조30지，분별접충1×106 ml-1、1×107 ml-1、1×108 ml-14T1유선암세포주세포현액。매조소서재수궤분량조분별접충우흉벽화측복벽。비교각조적성류시간、성류솔화8주존활솔，병용C-erbB-2면역조화법검사종류병리상태。결과：세포농도위1×107 ml-1시，종류성류솔고，생장은정；4 T1세포불동부위접충불영향성류솔급생장；C-erbB-2면역조직화학검사균위양성。결론：응용1×107 ml-1농도적4T1세포진행접충시최가적모형제작방법。
Objective:To establish a model of mice breast cancer of 4T1 cells in BALB/c mice and choose the best model making method.Methods:Ninety mice were divided into three groups randomly ,with 30 in each group injected by 4T1 cells suspension of 1×106 ml-1 ,1×107 ml-1 ,1×108 ml-1 respectively.Each group of mice were randomly divided into two groups which were inoculated on the chest wall and lateral abdominal wall respectively.Tumor formation time ,tumor growth rate and the 8 week survival rate in each group were compared ,and pathological character was observed by C-erbB-2 immunohistochemistry staining.Results:Tumor growth rate in cells suspension of 1×107 ml-1 was high and grow steadily.Tumor growth rate wasn′t correlative with 4T1 cells injection in different parts.The result of C-erbB-2 immunohistochemistry staining was positive.Conclusion: Injection of 4T1 cells suspension with 1 ×107 ml-1 is the best way to male tumor model in three suspension.