中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
6期
763-767
,共5页
钱志英%刘亚洁%崔艳品%谢李宁%张顺利
錢誌英%劉亞潔%崔豔品%謝李寧%張順利
전지영%류아길%최염품%사리저%장순리
水红花子%巨噬细胞%CD14+%CD204+%TNF-α%流产%小鼠
水紅花子%巨噬細胞%CD14+%CD204+%TNF-α%流產%小鼠
수홍화자%거서세포%CD14+%CD204+%TNF-α%유산%소서
Fructus-polygoni-orientalis%Macrophage%CD14+%CD204+%TNF-α%Abortion%Mouse
目的：探讨水红花子对细菌脂多糖（ LPS）诱导流产小鼠的免疫抑制及其保胎作用。方法：昆明种小鼠55只，随机分为对照组（A组，10只）和实验组。实验组又分为细菌脂多糖（LPS）处理组（B组），水红花子处理组（C组），水红花子及LPS双处理组（ D组），每组各15只。观察妊娠结果，用酶组织化学、免疫组化和ELISA方法分别检测非特异性酯酶阳性（α-NAE＋）、CD14＋和CD204＋巨噬细胞、TNF-α的变化。结果：B组小鼠流产率和胚胎吸收率均为100％（ P＜0.01）；而D组流产率降低为13.33％，胚胎吸收率降至10.39％。与A组相比，在B组，α-NAE＋、CD14＋巨噬细胞数量和阳性面积，以及TNF-α含量均显著增加；在D组，环肌外侧极显著增多（P＜0.01），但环肌内侧、功能层均接近正常水平。与A、B组相比，C、D组CD204＋巨噬细胞数量和阳性面积均显著增多。结论：通过调节子宫巨噬细胞的表型、活性和TNF-α的分泌，可能是水红花子对抗LPS所致流产效应的机制之一。
目的：探討水紅花子對細菌脂多糖（ LPS）誘導流產小鼠的免疫抑製及其保胎作用。方法：昆明種小鼠55隻，隨機分為對照組（A組，10隻）和實驗組。實驗組又分為細菌脂多糖（LPS）處理組（B組），水紅花子處理組（C組），水紅花子及LPS雙處理組（ D組），每組各15隻。觀察妊娠結果，用酶組織化學、免疫組化和ELISA方法分彆檢測非特異性酯酶暘性（α-NAE＋）、CD14＋和CD204＋巨噬細胞、TNF-α的變化。結果：B組小鼠流產率和胚胎吸收率均為100％（ P＜0.01）；而D組流產率降低為13.33％，胚胎吸收率降至10.39％。與A組相比，在B組，α-NAE＋、CD14＋巨噬細胞數量和暘性麵積，以及TNF-α含量均顯著增加；在D組，環肌外側極顯著增多（P＜0.01），但環肌內側、功能層均接近正常水平。與A、B組相比，C、D組CD204＋巨噬細胞數量和暘性麵積均顯著增多。結論：通過調節子宮巨噬細胞的錶型、活性和TNF-α的分泌，可能是水紅花子對抗LPS所緻流產效應的機製之一。
목적：탐토수홍화자대세균지다당（ LPS）유도유산소서적면역억제급기보태작용。방법：곤명충소서55지，수궤분위대조조（A조，10지）화실험조。실험조우분위세균지다당（LPS）처리조（B조），수홍화자처리조（C조），수홍화자급LPS쌍처리조（ D조），매조각15지。관찰임신결과，용매조직화학、면역조화화ELISA방법분별검측비특이성지매양성（α-NAE＋）、CD14＋화CD204＋거서세포、TNF-α적변화。결과：B조소서유산솔화배태흡수솔균위100％（ P＜0.01）；이D조유산솔강저위13.33％，배태흡수솔강지10.39％。여A조상비，재B조，α-NAE＋、CD14＋거서세포수량화양성면적，이급TNF-α함량균현저증가；재D조，배기외측겁현저증다（P＜0.01），단배기내측、공능층균접근정상수평。여A、B조상비，C、D조CD204＋거서세포수량화양성면적균현저증다。결론：통과조절자궁거서세포적표형、활성화TNF-α적분비，가능시수홍화자대항LPS소치유산효응적궤제지일。
Objective:To explore the immunosuppression effects and tocolysis of fructus polygoni orientalis on abortion mice induced by LPS.Methods:Mice of Kunming (55 mice) were divided into control group (group A ,10 mice) and experimental group. Experimental group were divided into group B ( intraperitoneal injection LPS ) , group C ( ingestion fructus polygoni orientalis ) , and group D (ingestion fructus polygoni orientalis and intraperitoneal injection LPS ),each group of 15 mice.Then the pregnancy results were observed ,the positive of α-NAE+and the varity of CD 14+and CD204+macrophages ,TNF-αin the uteri were identified by enzyme-histochemistry ,immunohistochemistry and ELISA.Results:The abortion rate and the embryo resorbing rate were all 100%( P<0.01 ) in group B.But there was the decreased abortion rate of 13.33% in group D .The embryo resorbing rate decreased to 10.39%.The number and positive cell area of α-NAE+and CD14+macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A ( P<0.01 ) .These effects outside of myometrium of group D were remarkably increased comparing with group A (P<0.01),but there was no distinct difference in the inside of myometrium and function layer.The number and positive cell area of CD204+macrophages in group C and D was greatly increased comparing with group A and B ( P<0.01 ) .The TNF-αcontents in the uteri of mice in group B were greatly increased comparing with group A (P<0.01),but the positive cell area of CD14,CD204 were close to normal levels in group D.Conclusion:The effect of miscarriage induced by LPS is antagonized by fructus polygoni orientalis through inhibiting the phenotype ,activity and function characteristics of macrophages in the uteri of gravidity mice.
