CAJ | 학술논문

目的：观察冠心舒通对脑缺血再灌注（ I/R）大鼠学习记忆能力及脑组织Bcl-2、Bax蛋白表达的影响，以探究冠心舒通对I/R损伤脑组织的保护机制。方法：以健康Wistar大鼠为研究对象，随机分为假手术组、脑I/R模型组、冠心舒通高剂量（1 g/kg）组和低剂量（0.5 g/kg）组，每组各15只。采用大鼠左侧颈总动脉结扎方法建立I/R模型，造模前每组分别每日灌胃给予生理盐水、生理盐水、冠心舒通高剂量和冠心舒通低剂量，连续7d；术后每天上午灌胃给予生理盐水、生理盐水、冠心舒通高剂量和冠心舒通低剂量，3 d后水迷宫训练同时继续给药，连续7 d，第8天进行Morris水迷宫测试；测试结束后处死大鼠取血液及脑组织，采用ELISA检测大鼠血清中Bcl-2和Bax的蛋白变化水平，采用HE染色观察大鼠脑组织的病理改变；采用免疫组织化学法检测大鼠脑组织中Bcl-2和Bax蛋白变化水平。结果：与假手术组比较，冠心舒通高剂量组大鼠测试潜伏期略延长、经过平台次数和平台象限停留时间略减少、血清及脑组织中Bax蛋白水平略升高、Bcl-2蛋白水平略降低但无显著差异（ P＞0.05），冠心舒通低剂量组大鼠测试潜伏期延长、经过平台次数和平台象限停留时间减少、血清及脑组织中Bax蛋白水平升高、Bcl-2蛋白水平降低有显著差异（ P＜0.01），I/R模型组大鼠测试潜伏期明显延长、经过平台次数和平台象限停留时间明显减少、血清及脑组织中Bax 蛋白水平明显升高、Bcl-2蛋白水平明显降低有显著差异（ P＜0.01）。结论：冠心舒通能明显提高Wistar大鼠空间记忆能力，并能通过降低Bax蛋白水平和提高Bcl-2蛋白水平，抑制脑I/R损伤后的细胞凋亡，从而对I/R脑组织具有保护作用。
목적：관찰관심서통대뇌결혈재관주（ I/R）대서학습기억능력급뇌조직Bcl-2、Bax단백표체적영향，이탐구관심서통대I/R손상뇌조직적보호궤제。방법：이건강Wistar대서위연구대상，수궤분위가수술조、뇌I/R모형조、관심서통고제량（1 g/kg）조화저제량（0.5 g/kg）조，매조각15지。채용대서좌측경총동맥결찰방법건립I/R모형，조모전매조분별매일관위급여생리염수、생리염수、관심서통고제량화관심서통저제량，련속7d；술후매천상오관위급여생리염수、생리염수、관심서통고제량화관심서통저제량，3 d후수미궁훈련동시계속급약，련속7 d，제8천진행Morris수미궁측시；측시결속후처사대서취혈액급뇌조직，채용ELISA검측대서혈청중Bcl-2화Bax적단백변화수평，채용HE염색관찰대서뇌조직적병리개변；채용면역조직화학법검측대서뇌조직중Bcl-2화Bax단백변화수평。결과：여가수술조비교，관심서통고제량조대서측시잠복기략연장、경과평태차수화평태상한정류시간략감소、혈청급뇌조직중Bax단백수평략승고、Bcl-2단백수평략강저단무현저차이（ P＞0.05），관심서통저제량조대서측시잠복기연장、경과평태차수화평태상한정류시간감소、혈청급뇌조직중Bax단백수평승고、Bcl-2단백수평강저유현저차이（ P＜0.01），I/R모형조대서측시잠복기명현연장、경과평태차수화평태상한정류시간명현감소、혈청급뇌조직중Bax 단백수평명현승고、Bcl-2단백수평명현강저유현저차이（ P＜0.01）。결론：관심서통능명현제고Wistar대서공간기억능력，병능통과강저Bax단백수평화제고Bcl-2단백수평，억제뇌I/R손상후적세포조망，종이대I/R뇌조직구유보호작용。
Objective:Guanxinshutong on cerebral ischemia reperfusion ( I/R) in rats learning and memory ability and brain Bcl-2,Bax protein expression to explore the protection mechanisms of Guanxinshutong on cerebral I /R injury in brain tissue.Methods:Healthy Wistar rats for the study were randomly divided into sham operation group ,I/R model group,high dose Guanxinshutong (1 g/kg) and low dose Guanxinshutong (0.5 g/kg) group,n=15.cerebral I/R model was estabhished by using the left commom cartid artery ligation method,modeling each group were fed daily before saline ,saline,Guanxinshutong (1 g/kg) and Guanxinshutong (0.5 g/kg), continuous 7 days;3 d after water maze training while continuing administration ,continuous 7 d,the eight day ,Morris water maze test;after the test,the rats were sacrificed blood and brain tissue ,serum by ELISA of Bcl-2 and Bax protein levels change;using HE staining of rat brain pathology;using immunohistochemistry assay in rat brain organization of Bcl-2 and Bax protein levels changes.Results:Compared with the sham group ,Guanxinshutong high-dose group test latency slightly longer timers after platform and platform quadrant dwell decreased slightly ,serum and brain tissue slightly elevated levels of Bax protein ,Bcl-2 protein levels decreased slightly but not significantly different ( P>0.05 ) ,low-dose group Guanxinshutong through latency test in rats ,after the number of platforms and platform quadrant dwell time decreased , serum and brain tissue elevated levels of Bax protein , Bcl-2 protein content decreased significantly different(P<0.01),cerebral I/R model group was significantly prolonged latency test ,after the number of platforms and platform quadrant dwell time significantly reduced serum and brain tissue was significantly increased Bax protein ,Bcl-2 protein content decreased with significant differences ( P<0.01 ).Conclusion: Guanxinshutong can significantly improve spatial memory , and by reducing Bax protein content and increased Bcl-2 protein,inhibition of cerebral I/R injury of apoptosis,and thus the brain tissue I/R has a protective effect.