中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2013年
8期
620-623
,共4页
朱仙花%陈锋%熊吉信%熊清根
硃仙花%陳鋒%熊吉信%熊清根
주선화%진봉%웅길신%웅청근
缺血%粒细胞集落刺激因子%纤维蛋白组织黏着剂
缺血%粒細胞集落刺激因子%纖維蛋白組織黏著劑
결혈%립세포집락자격인자%섬유단백조직점착제
Ischemia%Granulocyte colony-stimulating factor%Fibrin tissue adhesive
目的 观察纤维蛋白胶携载的粒细胞集落刺激因子(Gel+ G-CSF)对大鼠外周血白细胞总数和缺血下肢血管再生的影响.方法 雄性SD大鼠30只,制成右下肢缺血模型,随机分成3组:Gel+ G-CSF组、G-CSF组和PBS组,每组10只,分别肌肉注射Gel+ G-CSF、G-CSF和PBS.造模术前及术后第1、3、5、7天计数外周血白细胞总数,造模后第1周和第4周每组分别处死5只大鼠,行组织学检测,计数血管密度.结果 Gel+G-CSF组外周血白细胞总数在术后第1天达峰值[(14.69±1.11)×109/L],且明显低于G-CSF组在术后第3天达到的峰值[(21.00±2.26)×109/L].Gel+ G-CSF组[(686±108)/mm2]的毛细血管密度明显高于G-CSF组[(491±110)/mm2]和PBS组[(252±78)/mm2],P ＜0.05.Gel+ G-CSF组[(6.1±0.8)/mm2]的α-SMA阳性血管密度明显高于PBS组[(2.6±1.3)/mm2],P＜0.05.Gel+ G-CSF组肌组织中有大量血管内皮生长因子阳性细胞的浸润.结论 Gel+ G-CSF可以促进缺血肌组织血管再生,而刺激外周血白细胞计数升高的不良反应较小.
目的 觀察纖維蛋白膠攜載的粒細胞集落刺激因子(Gel+ G-CSF)對大鼠外週血白細胞總數和缺血下肢血管再生的影響.方法 雄性SD大鼠30隻,製成右下肢缺血模型,隨機分成3組:Gel+ G-CSF組、G-CSF組和PBS組,每組10隻,分彆肌肉註射Gel+ G-CSF、G-CSF和PBS.造模術前及術後第1、3、5、7天計數外週血白細胞總數,造模後第1週和第4週每組分彆處死5隻大鼠,行組織學檢測,計數血管密度.結果 Gel+G-CSF組外週血白細胞總數在術後第1天達峰值[(14.69±1.11)×109/L],且明顯低于G-CSF組在術後第3天達到的峰值[(21.00±2.26)×109/L].Gel+ G-CSF組[(686±108)/mm2]的毛細血管密度明顯高于G-CSF組[(491±110)/mm2]和PBS組[(252±78)/mm2],P ＜0.05.Gel+ G-CSF組[(6.1±0.8)/mm2]的α-SMA暘性血管密度明顯高于PBS組[(2.6±1.3)/mm2],P＜0.05.Gel+ G-CSF組肌組織中有大量血管內皮生長因子暘性細胞的浸潤.結論 Gel+ G-CSF可以促進缺血肌組織血管再生,而刺激外週血白細胞計數升高的不良反應較小.
목적 관찰섬유단백효휴재적립세포집락자격인자(Gel+ G-CSF)대대서외주혈백세포총수화결혈하지혈관재생적영향.방법 웅성SD대서30지,제성우하지결혈모형,수궤분성3조:Gel+ G-CSF조、G-CSF조화PBS조,매조10지,분별기육주사Gel+ G-CSF、G-CSF화PBS.조모술전급술후제1、3、5、7천계수외주혈백세포총수,조모후제1주화제4주매조분별처사5지대서,행조직학검측,계수혈관밀도.결과 Gel+G-CSF조외주혈백세포총수재술후제1천체봉치[(14.69±1.11)×109/L],차명현저우G-CSF조재술후제3천체도적봉치[(21.00±2.26)×109/L].Gel+ G-CSF조[(686±108)/mm2]적모세혈관밀도명현고우G-CSF조[(491±110)/mm2]화PBS조[(252±78)/mm2],P ＜0.05.Gel+ G-CSF조[(6.1±0.8)/mm2]적α-SMA양성혈관밀도명현고우PBS조[(2.6±1.3)/mm2],P＜0.05.Gel+ G-CSF조기조직중유대량혈관내피생장인자양성세포적침윤.결론 Gel+ G-CSF가이촉진결혈기조직혈관재생,이자격외주혈백세포계수승고적불량반응교소.
Objective To observe the effect of granulocyte colony-stimulating factor carried by fibrin gel on the total number of peripheral white blood cells and neovascularization of ischemic muscle in rat hindlimb ischemia model.Methods Thirty male SD rats were subjected to right hindlimb ischemia and randomly divided into three groups:Gel + G-CSF,G-CSF,and PBS,respectively injected with Gel+G-CSF,G-CSF and PBS.WBCs was detected before and 1,3,5,7 days; At first week and fourth week after surgery,5 rats in each group were sacrificed,and after histological detections was performed,blood vessels density counted.Results The peak value of WBCs counts in group Gel + G-CSF (14.69 ± 1.11 × 109/L)appeared at postoperative day 1 was significantly lower than that in group G-CSF (21.00 ±2.26 × 109/L) at postoperative day 3.The capillary density in group Gel + G-CSF (686 ± 108/mm2) was significantly higher than that in group G-CSF (491 ± 110/mm2) and group PBS (252 ± 78/mm2),P ＜ 0.05.The α-SMA-positive blood vessel density in group Gel + G-CSF (6.1 ± 0.8/mm2) was significantly higher than that in group PBS (2.6 ± 1.3/mm2),P ＜ 0.05.In group Gel + G-CSF,there were many VEGF-positive cells infiltrating in ischemic limb.Conclusions Gel + G-CSF promotes neovascularization in ischemic muscle,and induced more modest WBCs counts increase than the treatment with G-CSF.
