CAJ | 학술논문

大气压射频辉光放电（RF APGD）等离子体具有大气压下操作不需要真空系统、气体温度低、活性粒子浓度高、放电均匀性好、可控性强等特点，能够与各类生物分子发生作用，在生物技术中的应用受到广泛的关注。本研究团队将RF APGD等离子体射流引入生物诱变育种领域，对其物理特性及其与生物大分子和整细胞的作用机制进行了系统研究，并将其开发成新一代高效诱变育种仪，命名为常压室温等离子体（ARTP）诱变育种仪。实践证明，ARTP 诱变育种仪具有对操作者安全、环境友好、操作简便、突变快速、突变率高、获得的突变体性状稳定等特点，目前已成功应用于包括细菌、放线菌、真菌、酵母、微藻等在内的四十余种微生物的诱变育种。本文将对ARTP生物育种技术的最新研究进展进行综述，以期ARTP快速生物突变技术在生物进化研究及工业生物菌种改造上发挥重要作用。
대기압사빈휘광방전（RF APGD）등리자체구유대기압하조작불수요진공계통、기체온도저、활성입자농도고、방전균균성호、가공성강등특점，능구여각류생물분자발생작용，재생물기술중적응용수도엄범적관주。본연구단대장RF APGD등리자체사류인입생물유변육충영역，대기물리특성급기여생물대분자화정세포적작용궤제진행료계통연구，병장기개발성신일대고효유변육충의，명명위상압실온등리자체（ARTP）유변육충의。실천증명，ARTP 유변육충의구유대조작자안전、배경우호、조작간편、돌변쾌속、돌변솔고、획득적돌변체성상은정등특점，목전이성공응용우포괄세균、방선균、진균、효모、미조등재내적사십여충미생물적유변육충。본문장대ARTP생물육충기술적최신연구진전진행종술，이기ARTP쾌속생물돌변기술재생물진화연구급공업생물균충개조상발휘중요작용。
Radio-frequency atmospheric pressure glow discharge (RF APGD) plasmas have outstanding features of no need of expensive and complicated vacuum system, low gas temperatures, high concentrations of reactive species, good discharge uniformity, strong controllability and diverse interactions with various biomolecules, which have attracted more and more attentions for their applications in biotechnology. Our research group introduced the RF APGD plasma jet into the mutation breeding field. Based on the study on the physical characteristics of RF APGD plasmas and their interaction mechanisms with bio-macromolecules and whole cells, a novel mutation breeding system, named as atmospheric and room temperature plasma (ARTP) mutation breeding machine was invented. The ARTP mutation breeding system has the features of high safety for the operators and environment, easy operation, rapid mutation capability, high mutation rate and genetic stability of targeted mutants,as demonstrated by successful mutation for more than 40 different microbes including bacteria, fungi and algae. This paper reviews the recent research progress of ARTP mutation breeding technology, hopefully contributing to the development of the life evolution study and the industrial strain modification as a powerful mutation tool.