CAJ | 학술논문

酶催化具有化学、区位和立体选择性，这使得其成为绿色合成化学品的理想催化剂。然而，天然酶常因其在工业催化条件下的活性和稳定性较低而难以使用。纳米技术为构建高效酶催化剂提供了新的可能性。通过简便、高效、低成本的方法制备出具有高催化活性和高稳定性的纳米酶催化剂，同时提高纳米酶催化剂的可操作性和可回收重复使用特性是其中的关键问题。介绍了纳米酶催化剂的研究现状和制备方法，重点介绍了采用共沉淀方法制备酶-无机晶体杂化纳米催化剂、酶-金属有机骨架材料杂化纳米催化剂，以及制备具有温度和磁响应特性的纳米酶催化剂，并对纳米酶催化剂在酶催化合成医药化学品方面的应用前景进行了探讨。
매최화구유화학、구위화입체선택성，저사득기성위록색합성화학품적이상최화제。연이，천연매상인기재공업최화조건하적활성화은정성교저이난이사용。납미기술위구건고효매최화제제공료신적가능성。통과간편、고효、저성본적방법제비출구유고최화활성화고은정성적납미매최화제，동시제고납미매최화제적가조작성화가회수중복사용특성시기중적관건문제。개소료납미매최화제적연구현상화제비방법，중점개소료채용공침정방법제비매-무궤정체잡화납미최화제、매-금속유궤골가재료잡화납미최화제，이급제비구유온도화자향응특성적납미매최화제，병대납미매최화제재매최화합성의약화학품방면적응용전경진행료탐토。
The naturally evolved properties of enzyme catalysis in terms of chemo-, regio-, and stereo-selectivity make enzymes ideal catalysts for green synthesis of chemicals. However and unfortunately, low activity and poor stability frequently exclude enzymatic catalysis for industrial applications. The development of nanotechnology offers new opportunities to create highly efficient enzyme catalysts. The development of a facile, efficient and economical synthesis method and an easy way to operate and recycle the catalysts are the major challenges in this field. This review summarizes the recent advances in nanostructured enzyme catalysts, with the emphasis on the preparation of enzyme-inorganic hybrid nanocatalysts, enzyme metal-organic framework hybrid nanocatalysts, and the preparation of temperature-, or magnetic-responsive nanostructured enzyme catalysts. The potential applications of these nanostructured enzyme catalysts for enzymatic synthesis of pharmaceutical compounds are also discussed.