CAJ | 학술논문

目的：观察大鼠正畸牙移动过程中压力侧牙周组织NALP3的表达,探讨NALP3在正畸牙移动过程中的作用。方法将50只6周龄雄性SD大鼠随机分为10组,每组5只。以左上第一磨牙作为实验牙,分别加力0、1、3、6、12h、1、3、7、14、21d。制备不同时间牙周组织切片,进行HE染色和NALP3免疫组化染色。结果正畸牙移动过程中压力侧牙周组织NALP3的表达发生变化。加力后牙周膜NALP3表达即增加,3h达到小高峰,随后降低,1d后表达再次增加,7d后达到最高峰。结论 NALP3参与了正畸牙移动过程中牙周组织的改建。
목적：관찰대서정기아이동과정중압력측아주조직NALP3적표체,탐토NALP3재정기아이동과정중적작용。방법장50지6주령웅성SD대서수궤분위10조,매조5지。이좌상제일마아작위실험아,분별가력0、1、3、6、12h、1、3、7、14、21d。제비불동시간아주조직절편,진행HE염색화NALP3면역조화염색。결과정기아이동과정중압력측아주조직NALP3적표체발생변화。가력후아주막NALP3표체즉증가,3h체도소고봉,수후강저,1d후표체재차증가,7d후체도최고봉。결론 NALP3삼여료정기아이동과정중아주조직적개건。
Objective To examine the NALP3 expression in periodontal tissues of pressure side during orthodontic tooth movement in rats, and to explore the role of NALP3 during orthodontic tooth movement. Methods Fifty male SD rats aged 6 weeks old were randomly divided into 10 groups of 5. The upper left first molar was experimental tooth, which was pulled mesially by orthodontic force for 0, 1, 3, 6, 12 hours, 1, 3, 7, 14, 21 days respectively. Routine five-micrometer paraffin tissue sections were prepared and HE staining and immunohistochemical staining for NALP3 were carried out. Results The expression of NALP3 increased in pressure zone of periodontal ligament after loading, reaching a peak on the third hour and then decreased. The NALP3 expression increased again after one day, and reached the peak on the seventh day. Conclusion NALP3 was involved in the process of periodontal tissue remodeling during orthodontic tooth movement in rats.