中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
6期
421-423
,共3页
陈前明%方杰%瓦庆彪%袁伟%吴波%蔡琦%路永红%周培媚%蒋存火
陳前明%方傑%瓦慶彪%袁偉%吳波%蔡琦%路永紅%週培媚%蔣存火
진전명%방걸%와경표%원위%오파%채기%로영홍%주배미%장존화
皮肤肿瘤%基因,Pinx1%基因,hTERT
皮膚腫瘤%基因,Pinx1%基因,hTERT
피부종류%기인,Pinx1%기인,hTERT
Skin neoplasms%Genes,Pinx1%Genes,hTERT
目的 探讨内源性端粒酶抑制基因PinX1及端粒酶逆转录酶hTERT在基底细胞癌、鳞状细胞癌、日光性角化病组织中的表达及其意义.方法 实时定量PCR检测实验组36例3种皮肤肿瘤组织中内源性端粒酶抑制基因PinX1及端粒酶逆转录酶hTERT的mRNA表达水平,并以15例正常人皮肤组织作为对照组.结果 实验组PinX1基因的mRNA表达水平(2.53±1.57)低于对照组(4.25±2.02),差异有统计学意义(t=3.273,P＜ 0.05);hTERT基因的mRNA表达水平(6.37±3.81)明显高于对照组(1.62±0.99),差异有统计学意义(t=6.927,P＜0.05);Pinx1的表达水平与hTERT表达的相关性无统计学意义(P＞0.05).结论 PinX1的下调及hTERT上调可能与3种皮肤肿瘤形成过程中端粒酶激活及维持机制有关.
目的 探討內源性耑粒酶抑製基因PinX1及耑粒酶逆轉錄酶hTERT在基底細胞癌、鱗狀細胞癌、日光性角化病組織中的錶達及其意義.方法 實時定量PCR檢測實驗組36例3種皮膚腫瘤組織中內源性耑粒酶抑製基因PinX1及耑粒酶逆轉錄酶hTERT的mRNA錶達水平,併以15例正常人皮膚組織作為對照組.結果 實驗組PinX1基因的mRNA錶達水平(2.53±1.57)低于對照組(4.25±2.02),差異有統計學意義(t=3.273,P＜ 0.05);hTERT基因的mRNA錶達水平(6.37±3.81)明顯高于對照組(1.62±0.99),差異有統計學意義(t=6.927,P＜0.05);Pinx1的錶達水平與hTERT錶達的相關性無統計學意義(P＞0.05).結論 PinX1的下調及hTERT上調可能與3種皮膚腫瘤形成過程中耑粒酶激活及維持機製有關.
목적 탐토내원성단립매억제기인PinX1급단립매역전록매hTERT재기저세포암、린상세포암、일광성각화병조직중적표체급기의의.방법 실시정량PCR검측실험조36례3충피부종류조직중내원성단립매억제기인PinX1급단립매역전록매hTERT적mRNA표체수평,병이15례정상인피부조직작위대조조.결과 실험조PinX1기인적mRNA표체수평(2.53±1.57)저우대조조(4.25±2.02),차이유통계학의의(t=3.273,P＜ 0.05);hTERT기인적mRNA표체수평(6.37±3.81)명현고우대조조(1.62±0.99),차이유통계학의의(t=6.927,P＜0.05);Pinx1적표체수평여hTERT표체적상관성무통계학의의(P＞0.05).결론 PinX1적하조급hTERT상조가능여3충피부종류형성과정중단립매격활급유지궤제유관.
Objective To investigate the mRNA expressions of PinX1 and hTERT in lesions of three skin tumors (including basal cell carcinoma (BCC),squamous cell carcinoma (SCC) and actinic keratosis) as well as their significance.Methods This study included 36 tissue specimens from the lesions of 13 patients with BCC,11 patients with SCC and 12 patients with actinic keratosis,as well as 15 tissue specimens from the normal skin of 15 individuals receiving surgical therapy.Real-time PCR was performed to quantify the mRNA expressions of PinX1 and hTERT in these specimens.Results The specimens from the lesions of the skin tumors showed a significantly lower expression of PinX1 mRNA (2.53 ± 1.57 vs.4.25 ± 2.02,t =3.273,P ＜ 0.05),but a higher expression of hTERT mRNA (6.37 ± 3.81 vs.1.62 ± 0.99,t =6.927,P＜ 0.05) compared with those from the normal skin.There was no significant correlation between the mRNA expressions of PinX1 and hTERT (P ＞ 0.05).Conclusion The down-regulated PinX1 expression and up-regulated hTERT expression may be associated with the activation and maintenance of telomerase in the formation of the three skin tumors.
