中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
324-325,326
,共3页
杨柳%刘大鹏%张义生%周才新
楊柳%劉大鵬%張義生%週纔新
양류%류대붕%장의생%주재신
调经助孕颗粒%马钱苷%高效液相色谱
調經助孕顆粒%馬錢苷%高效液相色譜
조경조잉과립%마전감%고효액상색보
Tiaojing Zhuyun granules%Loganin%HPLC
目的：：建立调经助孕颗粒中马钱苷的含量测定方法。方法：采用高效液相色谱法,色谱柱为SinoChrom ODS-BP (250 mm ×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(14∶86),检测波长为240 nm,柱温30℃。结果：马钱苷进样量在50.40~1008.00 ng范围内与峰面积有良好的线性关系(r=0.9999),平均加样回收率为99.33%,RSD为1.41%(n=6)。结论：该方法快速、准确,操作简单,专属性强,可作为调经助孕颗粒中马钱苷的含量测定方法。
目的：：建立調經助孕顆粒中馬錢苷的含量測定方法。方法：採用高效液相色譜法,色譜柱為SinoChrom ODS-BP (250 mm ×4.6 mm,5μm),流動相為乙腈-0.1%燐痠水溶液(14∶86),檢測波長為240 nm,柱溫30℃。結果：馬錢苷進樣量在50.40~1008.00 ng範圍內與峰麵積有良好的線性關繫(r=0.9999),平均加樣迴收率為99.33%,RSD為1.41%(n=6)。結論：該方法快速、準確,操作簡單,專屬性彊,可作為調經助孕顆粒中馬錢苷的含量測定方法。
목적：：건립조경조잉과립중마전감적함량측정방법。방법：채용고효액상색보법,색보주위SinoChrom ODS-BP (250 mm ×4.6 mm,5μm),류동상위을정-0.1%린산수용액(14∶86),검측파장위240 nm,주온30℃。결과：마전감진양량재50.40~1008.00 ng범위내여봉면적유량호적선성관계(r=0.9999),평균가양회수솔위99.33%,RSD위1.41%(n=6)。결론：해방법쾌속、준학,조작간단,전속성강,가작위조경조잉과립중마전감적함량측정방법。
Objective:To establish a determination method for loganin in Tiaojing Zhuyun granules by HPLC. Methods: HPLC was used with a SinoChrom ODS-BP (250 mm × 4. 6 mm,5μm) as the analytical column. The mobile phase was composed of acetoni-trile-0. 1% phosphoric acid solution(14∶86). The detection wavelength was set at 240 nm and the column temperature was 30℃. Re-sults:The calibration curve of loganin was in a good linearity over the range of 50. 40-1 008. 00 ng(r=0. 999 9). The average recov-ery was 99. 33%(RSD=1. 41%, n=6). Conclusion:The method is simple,feasible and reproducible,and can be used in the quality control of Tiaojing Zhuyun granules.
