中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
316-318
,共3页
复方雷公藤糖浆%雷公藤甲素%高效液相色谱法
複方雷公籐糖漿%雷公籐甲素%高效液相色譜法
복방뢰공등당장%뢰공등갑소%고효액상색보법
Compound tripterygium wilfordil syrups%Triptolide%HPLC
目的：：建立高效液相色谱法测定复方雷公藤糖浆中雷公藤甲素的方法。方法：采用 Diamonsil C18(150 mm ×4.6 mm,5μm)色谱柱分离,甲醇-水(46∶54)为流动相,流速为1.0 ml·min-1,检测波长为224 nm,柱温为30℃,进样量为10μl。结果：雷公藤甲素在5.66~33.96μg ·ml-1(r=0.9998)范围内质量浓度与峰面积呈良好的线性关系,其平均回收率为99.35%(RSD=1.02%,n=9)。结论：本方法简便、灵敏、重复性好,可用于复方雷公藤糖浆中雷公藤甲素的质量控制。
目的：：建立高效液相色譜法測定複方雷公籐糖漿中雷公籐甲素的方法。方法：採用 Diamonsil C18(150 mm ×4.6 mm,5μm)色譜柱分離,甲醇-水(46∶54)為流動相,流速為1.0 ml·min-1,檢測波長為224 nm,柱溫為30℃,進樣量為10μl。結果：雷公籐甲素在5.66~33.96μg ·ml-1(r=0.9998)範圍內質量濃度與峰麵積呈良好的線性關繫,其平均迴收率為99.35%(RSD=1.02%,n=9)。結論：本方法簡便、靈敏、重複性好,可用于複方雷公籐糖漿中雷公籐甲素的質量控製。
목적：：건립고효액상색보법측정복방뢰공등당장중뢰공등갑소적방법。방법：채용 Diamonsil C18(150 mm ×4.6 mm,5μm)색보주분리,갑순-수(46∶54)위류동상,류속위1.0 ml·min-1,검측파장위224 nm,주온위30℃,진양량위10μl。결과：뢰공등갑소재5.66~33.96μg ·ml-1(r=0.9998)범위내질량농도여봉면적정량호적선성관계,기평균회수솔위99.35%(RSD=1.02%,n=9)。결론：본방법간편、령민、중복성호,가용우복방뢰공등당장중뢰공등갑소적질량공제。
Objective:To establish a method for the content determination of triptolide in compound tripterygium wilfordil syrups by HPLC. Methods:The samples were analyzed on a Diamonsil C18 column(150 mm × 4. 6 mm,5μm), the mobile phase consisted of methanol and water (46∶54), the flow rate was 1. 0 ml·min-1, the detection wavelength was 224 nm, and the column temperature was kept at 30℃, and the injection volume was 10 μl. Results: The linear range of triptolide was 5. 66-33. 96 μg · ml-1 ( r =0. 999 8), and the average recovery was 99. 35% with RSD of 1. 02%(n=9). Conclusion:The method is simple and sensitive with good repeatability, and can be used to control the quality of triptolide in compound tripterygium wilfordil syrups.
