中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
314-316
,共3页
马慧萍%李兰茹%董志臣%何蕾%贾正平
馬慧萍%李蘭茹%董誌臣%何蕾%賈正平
마혜평%리란여%동지신%하뢰%가정평
高效液相色谱法%安神益智胶囊%丹酚酸B%含量测定
高效液相色譜法%安神益智膠囊%丹酚痠B%含量測定
고효액상색보법%안신익지효낭%단분산B%함량측정
HPLC%Ansheng Yizhi capsules%Salvianolic acid B%Determination
目的：：建立高效液相色谱法测定安神益智胶囊中丹酚酸B的含量。方法：色谱柱为Hypersil ODS2 C18柱(150 mm ×4.6 mm,5μm),流动相为甲醇∶水∶甲酸(40∶60∶1),检测波长：286 nm；流速：1.0 ml·min-1；进样量：10μl。结果：丹酚酸B在7.75~77.51μg·ml-1的浓度范围内线性关系良好,r=0.9996,平均回收率为98.17%,RSD为1.79%(n=6)。结论：本法简便、准确,灵敏度高,重复性好,可作为控制安神益智胶囊质量的方法。
目的：：建立高效液相色譜法測定安神益智膠囊中丹酚痠B的含量。方法：色譜柱為Hypersil ODS2 C18柱(150 mm ×4.6 mm,5μm),流動相為甲醇∶水∶甲痠(40∶60∶1),檢測波長：286 nm；流速：1.0 ml·min-1；進樣量：10μl。結果：丹酚痠B在7.75~77.51μg·ml-1的濃度範圍內線性關繫良好,r=0.9996,平均迴收率為98.17%,RSD為1.79%(n=6)。結論：本法簡便、準確,靈敏度高,重複性好,可作為控製安神益智膠囊質量的方法。
목적：：건립고효액상색보법측정안신익지효낭중단분산B적함량。방법：색보주위Hypersil ODS2 C18주(150 mm ×4.6 mm,5μm),류동상위갑순∶수∶갑산(40∶60∶1),검측파장：286 nm；류속：1.0 ml·min-1；진양량：10μl。결과：단분산B재7.75~77.51μg·ml-1적농도범위내선성관계량호,r=0.9996,평균회수솔위98.17%,RSD위1.79%(n=6)。결론：본법간편、준학,령민도고,중복성호,가작위공제안신익지효낭질량적방법。
Objective:To establish an HPLC method for the content determination of salvianolic acid B in Ansheng Yizhi cap-sules. Methods:A Hypersil ODS2 C18 column(150 mm × 4. 6 mm,5 μm) was used and methanol-water-formic acid (40∶60∶1) was used as the mobile phase. The detection wavelength was at 286 nm. The flow rate was 1. 0 ml·min-1 and the sample size was 10 μl. Results:The calibration curve of salvianolic acid B was linear within the range 7. 75-77. 51 μg·ml-1(r=0. 999 6). The average re-covery was 98. 17%(RSD=1. 79%, n=6). Conclusion:The method is simple, accurate and repeatable, which can be used in the quality control of Ansheng Yizhi capsules.
