中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
312-314
,共3页
地胆草%4 ,5-二咖啡酰奎宁酸%高效液相色谱法
地膽草%4 ,5-二咖啡酰奎寧痠%高效液相色譜法
지담초%4 ,5-이가배선규저산%고효액상색보법
Elehantopus scaber Linn%4,5-Dicaffeoyquinic acid%HPLC
目的：：建立高效液相色谱法测定地胆草中4,5-二咖啡酰奎宁酸含量的方法。方法：色谱柱为Agilent SB C18柱(250 mm ×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(17∶83)；流速为1.0 ml·min-1；检测波长为327 nm；柱温为30℃,进样量10μl。结果：4,5-二咖啡酰奎宁酸在0.0235~2.3520μg范围内线性关系良好(r=0.9999),平均加样回收率为98.75%, RSD为1.24%(n=6)。结论：所用方法简便、快速、准确,可作为测定地胆草中4,5-二咖啡酰奎宁酸含量的方法。
目的：：建立高效液相色譜法測定地膽草中4,5-二咖啡酰奎寧痠含量的方法。方法：色譜柱為Agilent SB C18柱(250 mm ×4.6 mm,5μm),流動相為乙腈-0.1%燐痠溶液(17∶83)；流速為1.0 ml·min-1；檢測波長為327 nm；柱溫為30℃,進樣量10μl。結果：4,5-二咖啡酰奎寧痠在0.0235~2.3520μg範圍內線性關繫良好(r=0.9999),平均加樣迴收率為98.75%, RSD為1.24%(n=6)。結論：所用方法簡便、快速、準確,可作為測定地膽草中4,5-二咖啡酰奎寧痠含量的方法。
목적：：건립고효액상색보법측정지담초중4,5-이가배선규저산함량적방법。방법：색보주위Agilent SB C18주(250 mm ×4.6 mm,5μm),류동상위을정-0.1%린산용액(17∶83)；류속위1.0 ml·min-1；검측파장위327 nm；주온위30℃,진양량10μl。결과：4,5-이가배선규저산재0.0235~2.3520μg범위내선성관계량호(r=0.9999),평균가양회수솔위98.75%, RSD위1.24%(n=6)。결론：소용방법간편、쾌속、준학,가작위측정지담초중4,5-이가배선규저산함량적방법。
Objective:To establish an HPLC method for the determination of 4,5-dicaffeoyquinic acid in Elehantopus scaber Linn. Methods:The separation was carried out on an Agilent SB C18 column(250 mm × 4. 6 mm, 5 μm). The mobile phase was acetoni-trile-0. 1% phosphoric acid (17∶83) and the flow rate was 1. 0 ml·min-1 . The detection wavelength was set at 327nm, the column temperature was 30℃ and the injection volume was 10 μl. Results: The calibration curve showed a good linearity over the range of 0. 023 5-2. 352 0 μg(r=0. 999 9). The average recovery was 98. 75% with RSD of 1. 24%(n=6). Conclusion: The method is simple, rapid and accurate, and can be used as a quantitative analysis method for Elehantopus scaber Linn.
