中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
305-307
,共3页
鸦胆子油亚纳米乳注射液%急性毒性%长期毒性%小鼠%Beagle犬
鴉膽子油亞納米乳註射液%急性毒性%長期毒性%小鼠%Beagle犬
아담자유아납미유주사액%급성독성%장기독성%소서%Beagle견
Brucea javanica oil subnanoemulsion injections%Acute toxicity%Long-term toxicity%Mouse%Beagle dog
目的：：研究鸦胆子油亚纳米乳注射液的急性毒性和长期毒性。方法：以小鼠静脉注射,采用Bliss法计算其半数致死量(LD50)。 Beagle犬静脉注射鸦胆子油亚纳米乳注射液,分高、中、低三个剂量组(20,10,6 ml·kg-1),qd,连续8周,停药后恢复期3周,观察其对beagle犬的长期毒性反应及其程度。结果：小鼠静脉注射鸦胆子油亚纳米乳注射液的LD50为7.388 g· kg-1,95%可信限为6.306~8.656 g·kg-1。长期毒性试验中,三个剂量组的一般状况、体质量、血液学检查、血液生化学指标、心电图检查、脏器系数、脏器的肉眼观察和组织形态检查与对照组比较均无明显差异,但ALT和Cr值三个剂量组均有升高趋势,但无明显量效关系,停药3周复查,可完全恢复。结论：鸦胆子油亚纳米乳注射液毒性较低且病理变化具有可逆性,临床使用安全；但在用药期间应注意对肝肾功能的观察。
目的：：研究鴉膽子油亞納米乳註射液的急性毒性和長期毒性。方法：以小鼠靜脈註射,採用Bliss法計算其半數緻死量(LD50)。 Beagle犬靜脈註射鴉膽子油亞納米乳註射液,分高、中、低三箇劑量組(20,10,6 ml·kg-1),qd,連續8週,停藥後恢複期3週,觀察其對beagle犬的長期毒性反應及其程度。結果：小鼠靜脈註射鴉膽子油亞納米乳註射液的LD50為7.388 g· kg-1,95%可信限為6.306~8.656 g·kg-1。長期毒性試驗中,三箇劑量組的一般狀況、體質量、血液學檢查、血液生化學指標、心電圖檢查、髒器繫數、髒器的肉眼觀察和組織形態檢查與對照組比較均無明顯差異,但ALT和Cr值三箇劑量組均有升高趨勢,但無明顯量效關繫,停藥3週複查,可完全恢複。結論：鴉膽子油亞納米乳註射液毒性較低且病理變化具有可逆性,臨床使用安全；但在用藥期間應註意對肝腎功能的觀察。
목적：：연구아담자유아납미유주사액적급성독성화장기독성。방법：이소서정맥주사,채용Bliss법계산기반수치사량(LD50)。 Beagle견정맥주사아담자유아납미유주사액,분고、중、저삼개제량조(20,10,6 ml·kg-1),qd,련속8주,정약후회복기3주,관찰기대beagle견적장기독성반응급기정도。결과：소서정맥주사아담자유아납미유주사액적LD50위7.388 g· kg-1,95%가신한위6.306~8.656 g·kg-1。장기독성시험중,삼개제량조적일반상황、체질량、혈액학검사、혈액생화학지표、심전도검사、장기계수、장기적육안관찰화조직형태검사여대조조비교균무명현차이,단ALT화Cr치삼개제량조균유승고추세,단무명현량효관계,정약3주복사,가완전회복。결론：아담자유아납미유주사액독성교저차병리변화구유가역성,림상사용안전；단재용약기간응주의대간신공능적관찰。
Objective: To study the acute and long-term toxicity of brucea javanica oil subnanoemulsion injections ( BJOSI ) . Methods:The mice were given BJOSI by intravenous injection. The acute toxicity was observed and LD50 in mice was calculated by Bliss method. To observe the long-term toxicological effects, Beagle dogs were injected intravenously BJOSI once a day for 8-week du-ration with the dose of 20, 10 and 6 ml·kg-1 , respectively followed by 3-week recovery period. Results:LD50 of BJOSI in mice was 7. 388 g·kg-1 with 95% confidence limits of 6. 306-8. 656 g·kg-1 . The long term toxicity test showed that all the detected indices were within normal range in all groups, including general state, weight changes,hematological indices,biochemical indices,EEG,organ coefficients, morphological and histological changes, while there was an upward tendency of ALT and Cr in every BJOSI group without dose-effect correlation. The dogs could completely recover in three weeks after the administration. Conclusion:The results suggest that BJOSI has low toxicity,while the pathological changes are reversible, and attention should be paid to the function of liver and kidney.
