中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
238-240
,共3页
盐酸伐昔洛韦%胶囊%高效液相色谱法%含量测定
鹽痠伐昔洛韋%膠囊%高效液相色譜法%含量測定
염산벌석락위%효낭%고효액상색보법%함량측정
Valacyclovir hydrochloride%Capsules%HPLC%Content determination
目的：：建立测定盐酸伐昔洛韦胶囊含量的HPLC法。方法：色谱柱为Inertsil ODS (250 mm ×4.6 mm,5μm),以甲醇-0.02 mol·L-1磷酸二氢钾溶液(20∶80)为流动相,流速为1.0 ml·min-1,检测波长为251 nm,柱温30℃,进样量20μl。结果：盐酸伐昔洛韦在2~40μg·ml-1浓度范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.8%,RSD=0.16%(n=9)。结论：该检测方法简便、准确度高、专属性强,可用于盐酸伐昔洛韦胶囊含量测定。
目的：：建立測定鹽痠伐昔洛韋膠囊含量的HPLC法。方法：色譜柱為Inertsil ODS (250 mm ×4.6 mm,5μm),以甲醇-0.02 mol·L-1燐痠二氫鉀溶液(20∶80)為流動相,流速為1.0 ml·min-1,檢測波長為251 nm,柱溫30℃,進樣量20μl。結果：鹽痠伐昔洛韋在2~40μg·ml-1濃度範圍內與峰麵積呈良好的線性關繫(r=0.9999),平均迴收率為99.8%,RSD=0.16%(n=9)。結論：該檢測方法簡便、準確度高、專屬性彊,可用于鹽痠伐昔洛韋膠囊含量測定。
목적：：건립측정염산벌석락위효낭함량적HPLC법。방법：색보주위Inertsil ODS (250 mm ×4.6 mm,5μm),이갑순-0.02 mol·L-1린산이경갑용액(20∶80)위류동상,류속위1.0 ml·min-1,검측파장위251 nm,주온30℃,진양량20μl。결과：염산벌석락위재2~40μg·ml-1농도범위내여봉면적정량호적선성관계(r=0.9999),평균회수솔위99.8%,RSD=0.16%(n=9)。결론：해검측방법간편、준학도고、전속성강,가용우염산벌석락위효낭함량측정。
Objective:To develop an HPLC method for the determination of valacyclovir hydrochloride capsules. Methods: An Inertsil ODS (250 mm × 4. 6 mm,5μm) column was employed with methanol-0. 02 mol·L-1 potassium dihydrogen phosphate solution (20∶80) as the mobile phase, the flow rate was 1. 0 ml·min-1, the detection wavelength was at 251 nm, the column temperature was 30℃,and the sample size was 20μl. Results:The linear range of valacyclovir hydrochloride was 2-40μg·ml-1 , the correlation coeffi-cient was 0. 999 9 and the average recovery was 99. 8%(RSD=0. 16%,n=9). Conclusion:The analytical method is simple, accu-rate and special, which can be used in the content determination and quality control of valacyclovir hydrochloride capsules.
