CAJ | 학술논문

目的：：建立蛭芪康胶囊的质量控制方法。方法：采用薄层色谱法对制剂中的天麻蜜环菌粉、大黄、黄芪进行定性鉴别。采用3,5-二硝基水杨酸光度法(DNS)测定制剂多糖含量,采用Folin-酚法(Lowry法)测定制剂肽含量。结果：定性鉴别检出制剂中的天麻蜜环菌粉、大黄、黄芪；DNS法测定结果,多糖在6.412~32.060μg·ml-1的范围内呈良好的线性关系( r=0.9995),平均回收率为95.86%,RSD为0.86%(n=6)；Folin-酚法测定结果,肽在0.0597~0.2984 mg·ml-1范围内呈良好的线性关系(r=0.9990),平均回收率为100.3%,RSD为1.88%(n=6)。结论：本方法简便、准确,可作为该制剂的质控方法。
목적：：건립질기강효낭적질량공제방법。방법：채용박층색보법대제제중적천마밀배균분、대황、황기진행정성감별。채용3,5-이초기수양산광도법(DNS)측정제제다당함량,채용Folin-분법(Lowry법)측정제제태함량。결과：정성감별검출제제중적천마밀배균분、대황、황기；DNS법측정결과,다당재6.412~32.060μg·ml-1적범위내정량호적선성관계( r=0.9995),평균회수솔위95.86%,RSD위0.86%(n=6)；Folin-분법측정결과,태재0.0597~0.2984 mg·ml-1범위내정량호적선성관계(r=0.9990),평균회수솔위100.3%,RSD위1.88%(n=6)。결론：본방법간편、준학,가작위해제제적질공방법。
Objective:To establish the quality control of Zhiqikang capsules. Methods:TLC was used to identify Gastrodia tuder halimasch, rhubarb and Astragalus mongholicus in the preparations. A spectrophotometry method with 3, 5-dinitrosalicylic acid (DNS) was used to measure the polysaccharide content in Zhiqikang capsules. A spectrophotometry method with Forint phenol method ( Low-ry) was used to measure the peptide content in the capsules. Results:The linear range of polysaccharide was obtained between 6. 412 and 32. 060μg·ml-1(r=0. 999 5), the average recovery was 95. 86% and RSD was 0. 86%. The linear range of peptide was ob-tained between 0.059 7 and 0.298 4 mg·ml-1(r=0.999 0), the average recovery was 100.3% and RSD was 1.88%(n=6). Conclusion:The assay method is simple and accurate in the quality control of the preparations.