中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
218-220
,共3页
徐玥%蔡烽%张钦%洪婷%徐燕丰
徐玥%蔡烽%張欽%洪婷%徐燕豐
서모%채봉%장흠%홍정%서연봉
康视明合剂%薄层色谱法%高效液相色谱法%质量标准
康視明閤劑%薄層色譜法%高效液相色譜法%質量標準
강시명합제%박층색보법%고효액상색보법%질량표준
Kangshiming mixtures%TLC%HPLC%Quality standard
目的::制定康视明合剂质量控制方法。方法:采用薄层色谱法( TLC)鉴别处方中的当归、黄柏、黄芪;采用高效液相色谱法( HPLC)对处方中的葛根素及芍药苷含量进行定量测定。结果:TLC法能定性检出当归、黄柏、黄芪,斑点清晰,且阴性对照无干扰。定量分析中葛根素和芍药苷分别在1.64~49.20μg·ml-1(r=0.9999)和4.22~63.30μg·ml-1(r=0.9996)范围内与峰面积呈良好线性,葛根素平均回收率为99.63%,RSD=2.14%(n=9);芍药苷平均回收率为99.05%,RSD=2.70%(n=9)。结论:本方法准确、可靠、专属性强,可用于康视明合剂的质量控制。
目的::製定康視明閤劑質量控製方法。方法:採用薄層色譜法( TLC)鑒彆處方中的噹歸、黃柏、黃芪;採用高效液相色譜法( HPLC)對處方中的葛根素及芍藥苷含量進行定量測定。結果:TLC法能定性檢齣噹歸、黃柏、黃芪,斑點清晰,且陰性對照無榦擾。定量分析中葛根素和芍藥苷分彆在1.64~49.20μg·ml-1(r=0.9999)和4.22~63.30μg·ml-1(r=0.9996)範圍內與峰麵積呈良好線性,葛根素平均迴收率為99.63%,RSD=2.14%(n=9);芍藥苷平均迴收率為99.05%,RSD=2.70%(n=9)。結論:本方法準確、可靠、專屬性彊,可用于康視明閤劑的質量控製。
목적::제정강시명합제질량공제방법。방법:채용박층색보법( TLC)감별처방중적당귀、황백、황기;채용고효액상색보법( HPLC)대처방중적갈근소급작약감함량진행정량측정。결과:TLC법능정성검출당귀、황백、황기,반점청석,차음성대조무간우。정량분석중갈근소화작약감분별재1.64~49.20μg·ml-1(r=0.9999)화4.22~63.30μg·ml-1(r=0.9996)범위내여봉면적정량호선성,갈근소평균회수솔위99.63%,RSD=2.14%(n=9);작약감평균회수솔위99.05%,RSD=2.70%(n=9)。결론:본방법준학、가고、전속성강,가용우강시명합제적질량공제。
Objective:To improve the quality standard for Kangshiming mixtures. Methods:The identification of Angelicae Sinen-sis Radix, Astragali Radix and Phellodendri Chinensis Cortex was carried out by TLC. The contents of puerarin and paeoniflorin in the preparations were determined by HPLC. Results:The spots displayed in TLC were clear without interference from the negative control. The linear range for puerarin was 1. 64-49. 20μg·ml-1(r=0. 999 9) and 4. 22-63. 30μg·ml-1(r=0. 999 6) for paeoniflorin. The average recovery was 99. 63%(RSD=2. 14%,n=9) and 99. 05%(RSD=2. 70%,n=9) for puerarin and paeoniflorin, respectively. Conclusion:The method is accurate, reliable and specific, and can be used in the quality control of Kangshiming mixtures.