中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
204-206
,共3页
徐娜%纪宏宇%唐景玲%吴琳华
徐娜%紀宏宇%唐景玲%吳琳華
서나%기굉우%당경령%오림화
染料木素%丙烯酸树脂%纳米粒%高效液相色谱%含量%包封率
染料木素%丙烯痠樹脂%納米粒%高效液相色譜%含量%包封率
염료목소%병희산수지%납미립%고효액상색보%함량%포봉솔
Genistein%Eudragit%Nanoparticles%HPLC%Content%Encapsulation efficiency
目的：：建立测定丙烯酸树脂纳米粒中染料木素含量的 HPLC 方法。方法：色谱柱：DiamonsilTM C18柱(250 mm ×4.6 mm,5μm)；流动相：甲醇-水=85∶15(v∶v)；流速：1.0 ml·min-1；柱温：25℃；检测波长：261 nm；进样量：10μl。结果：药物峰与辅料峰分离度良好,染料木素浓度在1.0~100.0μg·ml-1范围内线性关系良好(r=0.9998),平均回收率为99.85%(RSD=0.65%,n=9)。结论：本方法简便,快捷,准确度高,专属性强,适用于测定染料木素丙烯酸树脂纳米粒的含量及包封率。
目的：：建立測定丙烯痠樹脂納米粒中染料木素含量的 HPLC 方法。方法：色譜柱：DiamonsilTM C18柱(250 mm ×4.6 mm,5μm)；流動相：甲醇-水=85∶15(v∶v)；流速：1.0 ml·min-1；柱溫：25℃；檢測波長：261 nm；進樣量：10μl。結果：藥物峰與輔料峰分離度良好,染料木素濃度在1.0~100.0μg·ml-1範圍內線性關繫良好(r=0.9998),平均迴收率為99.85%(RSD=0.65%,n=9)。結論：本方法簡便,快捷,準確度高,專屬性彊,適用于測定染料木素丙烯痠樹脂納米粒的含量及包封率。
목적：：건립측정병희산수지납미립중염료목소함량적 HPLC 방법。방법：색보주：DiamonsilTM C18주(250 mm ×4.6 mm,5μm)；류동상：갑순-수=85∶15(v∶v)；류속：1.0 ml·min-1；주온：25℃；검측파장：261 nm；진양량：10μl。결과：약물봉여보료봉분리도량호,염료목소농도재1.0~100.0μg·ml-1범위내선성관계량호(r=0.9998),평균회수솔위99.85%(RSD=0.65%,n=9)。결론：본방법간편,쾌첩,준학도고,전속성강,괄용우측정염료목소병희산수지납미립적함량급포봉솔。
Objective:To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods:The HPLC analysis was carried out on a DiamonsilTM C18(250 mm ×4.6 mm,5μm)column with the mobile phase of meth-anol-water (85∶15) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 261nm and the column temperature was 25℃. Results: A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1(r =0.999 8). The recovery of genistein was 99.85%(RSD =0. 65%,n=9). Conclusion:The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles.
