中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
201-204
,共4页
固相萃取%高效液相色谱法%中成药%合成色素
固相萃取%高效液相色譜法%中成藥%閤成色素
고상췌취%고효액상색보법%중성약%합성색소
Solid phase extraction%HPLC%Chinese patent drugs%Synthetic pigments
目的：：建立检测中成药中人工合成色素的固相萃取-高效液相色谱( SPE-HPLC)分析方法。方法：样品利用聚酰胺固相萃取柱净化和富集后进行HPLC分析。采用资生堂C18色谱柱,以甲醇和0.02 mol·L-1醋酸铵为流动相进行梯度洗脱,检测波长：508 nm,柱温：30℃,进样量：10μl。结果：合成色素线性范围均为0.005~0.200μg(r =0.9999),回收率分别为97.6%(RSD=1.3%,n=6)、97.0%(RSD=1.4%,n=6)和99.5%(RSD=1.4%,n=6)。结论：该方法操作简单,结果准确可靠,重复性好,适用于中成药中人工合成色素的检测。
目的：：建立檢測中成藥中人工閤成色素的固相萃取-高效液相色譜( SPE-HPLC)分析方法。方法：樣品利用聚酰胺固相萃取柱淨化和富集後進行HPLC分析。採用資生堂C18色譜柱,以甲醇和0.02 mol·L-1醋痠銨為流動相進行梯度洗脫,檢測波長：508 nm,柱溫：30℃,進樣量：10μl。結果：閤成色素線性範圍均為0.005~0.200μg(r =0.9999),迴收率分彆為97.6%(RSD=1.3%,n=6)、97.0%(RSD=1.4%,n=6)和99.5%(RSD=1.4%,n=6)。結論：該方法操作簡單,結果準確可靠,重複性好,適用于中成藥中人工閤成色素的檢測。
목적：：건립검측중성약중인공합성색소적고상췌취-고효액상색보( SPE-HPLC)분석방법。방법：양품이용취선알고상췌취주정화화부집후진행HPLC분석。채용자생당C18색보주,이갑순화0.02 mol·L-1작산안위류동상진행제도세탈,검측파장：508 nm,주온：30℃,진양량：10μl。결과：합성색소선성범위균위0.005~0.200μg(r =0.9999),회수솔분별위97.6%(RSD=1.3%,n=6)、97.0%(RSD=1.4%,n=6)화99.5%(RSD=1.4%,n=6)。결론：해방법조작간단,결과준학가고,중복성호,괄용우중성약중인공합성색소적검측。
Objective:To establish a method for detecting synthetic pigments in Chinese patent drugs by solid phase extraction-HPLC(SPE-HPLC). Methods:Purified with SPE and concentrated, the sample was separated on a SHISEIDO C18 column with 0. 02 mol·L-1 ammonium acetate and methanol mixed solution as the mobile phase with gradient elution at the flow rate of 1. 0 ml·min-1 , the detection wavelength of 508nm, column temperature of 30℃,and the injection volume of 10μl. Results: The linear range of the synthetic pigments was 0. 005-0. 200 μg(r=0. 999 9), and the average recovery was 97. 6%(RSD=1. 3%,n=6),97. 0%(RSD=1. 4%,n=6) and 99. 5%(RSD=1. 4%,n=6), respectively. Conclusion:The proposed method is simple, reliable and reproduci-ble, and especially suitable for the detection of synthetic pigments in Chinese patent drugs.
