CAJ | 학술논문

目的：：建立HPLC法测定血浆中华法林浓度方法,并用此方法监测本院术后患者华法林血浓度。方法：采用ZORB-AX Eclipse XDB-C8色谱柱(150 mm ×4.6 mm,5μm),流动相：0.1%醋酸：甲醇(35∶65),流速：1.0 ml·min-1；检测波长：308 nm；柱温：30℃；进样量：20μl。二极管阵列检测器检测。结果：线性范围为0.1~5.12μg · ml-1,提取回收率为85.0%~102.3%,RSD为1.2%~3.9%(n=6)。日内、日间RSD均小于5%,重复性好。结论：该方法准确、简便、精密度高,可用于华法林血药浓度监测。
목적：：건립HPLC법측정혈장중화법림농도방법,병용차방법감측본원술후환자화법림혈농도。방법：채용ZORB-AX Eclipse XDB-C8색보주(150 mm ×4.6 mm,5μm),류동상：0.1%작산：갑순(35∶65),류속：1.0 ml·min-1；검측파장：308 nm；주온：30℃；진양량：20μl。이겁관진렬검측기검측。결과：선성범위위0.1~5.12μg · ml-1,제취회수솔위85.0%~102.3%,RSD위1.2%~3.9%(n=6)。일내、일간RSD균소우5%,중복성호。결론：해방법준학、간편、정밀도고,가용우화법림혈약농도감측。
Objective:To establish a simple HPLC method for the determination of warfarin in human plasma and analyze the re-sults of 100 monitoring reports. Methods:Warfarin was extracted from the plasma samples by acetonitrile. The extract was separated on a ZORBAX Eclipse XDB-C8 column (150 mm × 4. 6 mm, 5 μm)with a mobile phase consisting of methanol and 0. 1% acetic acid (65∶35) at flow rate of 1. 0 ml·min-1 and detected by a diode array detector. The detection wavelength was at 308nm and the sample size was 20 μl. Results:The calibration curve was linear within the range of 0. 1-5. 12μg·ml-1 . The average recovery and RSD was ranged from 85. 0% to 102. 3% and 1. 2% to 3. 9%(n=6), respectively. Inter-and intra-day RSD were 1. 1%-1. 8% and 1. 6%-2. 9%, respectively. Conclusion:The proposed method is rapid, convenient and accurate in the detection of warfarinin concentration in plasma.