CAJ | 학술논문

目的：探讨未成熟树突状细胞（imDC）吞噬经补骨脂素长波紫外线（PUVA）处理的小鼠脾B淋巴细胞（PUVA-SP）后，将B淋巴细胞诱导为高分泌白细胞介素（IL）-10（IL-10＋）的调节性B细胞的可行性。方法培养小鼠骨髓来源DC，小鼠脾淋巴细胞经PUVA光源照射得到PUVA-SP，在体外将 PUVA-SP 与骨髓来源的 imDC 共同培养，后者吞噬 PUVA-SP 后形成 PUVA-SP DC。应用CD19磁珠分选小鼠脾脏B淋巴细胞，将不同的DC与B淋巴细胞共同培养48 h。采用酶链免疫吸附试验（ELISA）检测B淋巴细胞、imDC、imDC＋B淋巴细胞、PUVA-SP DC、PUVA-SP DC＋B淋巴细胞的培养上清液中干扰素（IFN）-γ、转化生长因子（TGF）-β、IL-12p70、IL-10等细胞因子的含量。流式细胞仪分析 B 淋巴细胞、imDC ＋B 淋巴细胞、mDC ＋B 淋巴细胞、PUVA-SP DC ＋B淋巴细胞共培养后IL-10＋调节性B细胞数量。结果与其他4组细胞培养液比较，PUVA-SP DC＋B淋巴细胞上清液中的IL-10含量明显升高（均为P＜0.05）。与其他各组相比，PUVA-SP DC＋B淋巴细胞中IL-10＋调节性B细胞数量明显增多。结论 PUVA-SP DC可以诱导脾脏B淋巴细胞分化为IL-10＋调节性B细胞。
목적：탐토미성숙수돌상세포（imDC）탄서경보골지소장파자외선（PUVA）처리적소서비B림파세포（PUVA-SP）후，장B림파세포유도위고분비백세포개소（IL）-10（IL-10＋）적조절성B세포적가행성。방법배양소서골수래원DC，소서비림파세포경PUVA광원조사득도PUVA-SP，재체외장 PUVA-SP 여골수래원적 imDC 공동배양，후자탄서 PUVA-SP 후형성 PUVA-SP DC。응용CD19자주분선소서비장B림파세포，장불동적DC여B림파세포공동배양48 h。채용매련면역흡부시험（ELISA）검측B림파세포、imDC、imDC＋B림파세포、PUVA-SP DC、PUVA-SP DC＋B림파세포적배양상청액중간우소（IFN）-γ、전화생장인자（TGF）-β、IL-12p70、IL-10등세포인자적함량。류식세포의분석 B 림파세포、imDC ＋B 림파세포、mDC ＋B 림파세포、PUVA-SP DC ＋B림파세포공배양후IL-10＋조절성B세포수량。결과여기타4조세포배양액비교，PUVA-SP DC＋B림파세포상청액중적IL-10함량명현승고（균위P＜0.05）。여기타각조상비，PUVA-SP DC＋B림파세포중IL-10＋조절성B세포수량명현증다。결론 PUVA-SP DC가이유도비장B림파세포분화위IL-10＋조절성B세포。
Objective To investigate the feasibility of immature dendritic cells (imDC)phagocytized psoralen ultraviolet A (PUVA)-treated splenic lymphocytes (PUVA-SP DC)in mice inducing B lymphocytes to be regulatory B cells (Breg)with high secretion of interleukin (IL)-10 (IL-10 +Breg). Methods Bone marrow-derived DC of mice was cultured. Spleen lymphocytes of mice were isolated and treated by PUVA,and turned to be PUVA-SP. The bone marrow-derived imDC was co-cultured with PUVA-SP in vitro to obtain PUVA-SP DC. Splenic B lymphocytes of mice were separated by anti-CD19 magnetic beads and co-cultured with different kinds of DC for 48 hours. The levels of interferon (IFN )-γ,transforming growth factor (TGF)-β,IL-12p70,and IL-10 in the culture supernatant of B lymphocytes,imDC,imDC+B lymphocytes, PUVA-SP DC and PUVA-SP DC +B lymphocytes were measured by enzyme-linked immune absorbent assay (ELISA). The accounts of IL-10 +Breg in B lymphocytes,imDC+B lymphocytes,mDC+B lymphocytes and PUVA-SP DC+B lymphocytes were detected by flow cytometry. Results Compared with the other 4 groups, the level of IL-10 in cell culture supernatant of PUVA-SP DC+B lymphocytes was significantly higher (all in P<0.05). Compared with the other groups,the account of IL-10 +Breg in PUVA-SP DC+B lymphocytes was significantly higher. Conclusions PUVA-SP DC can induce splenic B lymphocytes to differentiate into IL-10 +Breg.