CAJ | 학술논문

背景：前期研究已明确三七总皂苷能抑制乙醇诱导的兔骨髓基质干细胞成脂分化，证实三七总皂苷能够促进兔成骨细胞的增殖和分化，并能提高成骨细胞的骨保护素 mRNA 的相对表达量而对其细胞核因子 kB 受体活化因子配体 mRNA 有抑制作用。 　　目的：进一步观察三七总皂苷对兔酒精性股骨头缺血坏死超微结构的影响。 　　方法：白酒灌胃6周制备新西兰兔股骨头酒精性缺血坏死模型，造模成功的模型兔分别于股骨头局部注射生理盐水、复方骨肽及三七总皂苷，注射量为0.1 mL/kg，每周给药1次，连续治疗4周，通过透射电镜观察各组超微结构变化。 　　结果与结论：透射电镜显示，酒精性缺血坏死骨细胞线粒体肿胀，嵴结构模糊，粗面内质网上多聚核糖体脱颗粒，骨细胞内可见脂滴出现。与生理盐水组相比，三七总皂苷组骨细胞线粒体肿胀消退，可见到嵴结构，粗面内质网上多聚核糖体增多，脂滴数量明显减少。复方骨肽组超微结构形态改变与三七总皂苷组相似。三七组和复方骨肽组骨细胞超微结构形态变化明显好于生理盐水组(P<0.05)。实验证实三七总皂苷可有效促进早期兔酒精性股骨头缺血性坏死骨细胞超微结构的修复。
배경：전기연구이명학삼칠총조감능억제을순유도적토골수기질간세포성지분화，증실삼칠총조감능구촉진토성골세포적증식화분화，병능제고성골세포적골보호소 mRNA 적상대표체량이대기세포핵인자 kB 수체활화인자배체 mRNA 유억제작용。 　　목적：진일보관찰삼칠총조감대토주정성고골두결혈배사초미결구적영향。 　　방법：백주관위6주제비신서란토고골두주정성결혈배사모형，조모성공적모형토분별우고골두국부주사생리염수、복방골태급삼칠총조감，주사량위0.1 mL/kg，매주급약1차，련속치료4주，통과투사전경관찰각조초미결구변화。 　　결과여결론：투사전경현시，주정성결혈배사골세포선립체종창，척결구모호，조면내질망상다취핵당체탈과립，골세포내가견지적출현。여생리염수조상비，삼칠총조감조골세포선립체종창소퇴，가견도척결구，조면내질망상다취핵당체증다，지적수량명현감소。복방골태조초미결구형태개변여삼칠총조감조상사。삼칠조화복방골태조골세포초미결구형태변화명현호우생리염수조(P<0.05)。실험증실삼칠총조감가유효촉진조기토주정성고골두결혈성배사골세포초미결구적수복。
BACKGROUND:Previous studies have demonstrated that total saponins of panax notoginseng can inhibit the ethanol-induced adipogenic differentiation of rabbit bone marrow stromal cells and confirmed that total saponins of panax notoginseng promoted the proliferation and differentiation of rabbit osteoblasts, and improved the osteoprotegerin mRNA relative expression in osteoblasts so as to inhibit receptor activator of nuclear factor kappa B ligand mRNA expression. OBJECTIVE:To observe effects of total saponins of panax notoginseng on the ultrastructure of the rabbit models of alcoholic avascular necrosis of the femoral head. METHODS:New Zealand rabbit models of alcohol-induced avascular necrosis of the femoral head were established by gavage of spirit. Successful rabbit models were separately injected with saline, compound bone peptide and total saponins of panax notoginseng group, 0.1 mL/kg, once a day, for 4 consecutive weeks. The ultrastructure of each group were observed by transmission electron microscope. RESULTS AND CONCLUSON:Transmission electron microscopy showed that osteocytes after alcoholic avascular necrosis of the femoral head presented mitochondrial swel ing and fuzzy crista structure, and degranulation of polysomes on rough endoplasmic reticulum. Lipid droplets were seen in osteocytes. Compared with saline group, mitochondria swel ing subsided, cristae appeared, the number of polysomes increased on rough endoplasmic reticulum, but the number of lipid droplet decreased in total saponins of panax notoginseng group. Morphological changes in ultrastructure were similar between compound bone peptide group and total saponins of panax notoginseng group. Morphological changes in ultrastructure were more significant in the total saponins of panax notoginseng and compound bone peptide groups compared with saline group (P<0.05). Results verified that total saponins of panax notoginseng could effectively restore ultrastructure of osteocytes of rabbit models of alcoholic avascular necrosis of the femoral head in the early stage.