CAJ | 학술논문

背景：菟丝子是旋花科植物菟丝子Cuscutachinensis Lam.的成熟种子，为温补肾阳的要药，前期研究表明，由菟丝子组成的补肾复方在抑制骨量丢失，改善骨密度方面有明显疗效。 　　目的：探讨菟丝子黄酮对去卵巢骨质疏松模型大鼠股骨骨密度、血清和肾脏1，25-二羟基维生素D3(1，25(OH)2D3)含量、小肠钙结合蛋白(CaBp-D9K)mRNA表达的影响。 　　方法：72只SD雌性大鼠，随机数字表法均分为6组(n=12)：假手术组、模型组、维生素 D3组和菟丝子黄酮小、中、大剂量组。假手术组仅行假手术，其余5组分别行卵巢切除，1周后分别灌胃给予维生素D3(2 mg/kg)以及小、中、大剂量菟丝子黄酮连续给药3个月。腹主动脉取血，分离血清，取出肾脏，采用酶联免疫吸附法检测1，25(OH)2D3含量。之后处死动物，取出股骨，测定骨密度；取出第2腰椎，采用实时荧光反转录聚合酶链反应(real-time RT-PCR)测定腰椎和肾脏组织维生素D受体mRNA表达。取出小肠，采用RT-PCR测定小肠CaBp-D9K mRNA表达。 　　结果与结论：与假手术组相比，模型组股骨骨密度、血清和肾脏1，25(OH)2D3、腰椎组织维生素D受体mRNA、小肠CaBp-D9K mRNA表达均下降。与模型组比较，菟丝子黄酮中、大剂量组和维生素D3组均可使股骨骨密度、血清和肾脏1，25(OH)2D3、腰椎组织维生素D受体mRNA、小肠CaBp-D9K mRNA表达增加。菟丝子黄酮能够显著提高去卵巢大鼠股骨骨密度、血清和肾脏1，25(OH)2D3、腰椎组织维生素D受体mRNA、小肠CaBp-D9K mRNA表达，促进肠钙吸收与成骨细胞活性，增强骨质量。
배경：토사자시선화과식물토사자Cuscutachinensis Lam.적성숙충자，위온보신양적요약，전기연구표명，유토사자조성적보신복방재억제골량주실，개선골밀도방면유명현료효。 　　목적：탐토토사자황동대거란소골질소송모형대서고골골밀도、혈청화신장1，25-이간기유생소D3(1，25(OH)2D3)함량、소장개결합단백(CaBp-D9K)mRNA표체적영향。 　　방법：72지SD자성대서，수궤수자표법균분위6조(n=12)：가수술조、모형조、유생소 D3조화토사자황동소、중、대제량조。가수술조부행가수술，기여5조분별행란소절제，1주후분별관위급여유생소D3(2 mg/kg)이급소、중、대제량토사자황동련속급약3개월。복주동맥취혈，분리혈청，취출신장，채용매련면역흡부법검측1，25(OH)2D3함량。지후처사동물，취출고골，측정골밀도；취출제2요추，채용실시형광반전록취합매련반응(real-time RT-PCR)측정요추화신장조직유생소D수체mRNA표체。취출소장，채용RT-PCR측정소장CaBp-D9K mRNA표체。 　　결과여결론：여가수술조상비，모형조고골골밀도、혈청화신장1，25(OH)2D3、요추조직유생소D수체mRNA、소장CaBp-D9K mRNA표체균하강。여모형조비교，토사자황동중、대제량조화유생소D3조균가사고골골밀도、혈청화신장1，25(OH)2D3、요추조직유생소D수체mRNA、소장CaBp-D9K mRNA표체증가。토사자황동능구현저제고거란소대서고골골밀도、혈청화신장1，25(OH)2D3、요추조직유생소D수체mRNA、소장CaBp-D9K mRNA표체，촉진장개흡수여성골세포활성，증강골질량。
BACKGROUND:Cuscuta chinensis is a mature seed of Cuscutachinensis Lam., can warm kidney. Previous studies demonstrated that kidney compound composed of Cuscuta chinensis could apparently inhibit bone loss and improve bone density. OBJECTIVE:To explore the influence of flavonoids from Cuscuta chinensis on bone mineral density of femur, 1,25(OH)2D3 levels of serum and kidney, the expression of smal intestine CaBp-D9K mRNA in model rats with ovariectomized osteoporosis. METHODS:A total of 72 Sprague-Dawley female rats were equal y and randomly divided into six groups (n=12):sham surgery group, model group, vitamin D3 group and low-, moderate-and high-dose flavonoids groups. The sham surgery group only received sham operation and the other five groups were ovariectomized respectively. One week after ovariectomy, the rats were given flavonoids from low-, moderate-and high-dose Cuscuta chinensis and vitamin D3 (2 mg/kg) by intragastric administration for 3 consecutive months. Blood was obtained from the abdominal aorta. Serum was isolated. The kidney was obtained. Enzyme linked immunosorbent assay was used to determine 1,25(OH)2D3 contents of renal and serum. Rats were sacrificed at the end of experiment. The thighbone was taken out to determine bone mineral density. The second lumbar vertebra was taken out to measure the expression of lumbar vertebra and renal vitamin D receptor mRNA using real-time fluorescent reverse transcription-polymerase chain reaction. The smal intestine was taken out to measure the expression of smal intestine CaBp-D9K mRNA using real-time fluorescent reverse transcription-polymerase chain reaction. RESULTS AND CONCLUSION:Compared with the sham operation group, bone mineral density of femur, and 1,25(OH)2D3 levels of serum and kidney and the expression of lumbar vertebra vitamin D receptor mRNA significantly decreased in model group, and the expression of smal intestine CaBp-D9K mRNA significantly decreased in model group. Compared with the model group, bone mineral density of femur, and 1,25(OH)2D3 levels of serum and kidney, and the expression of the second lumbar vertebra vitamin D receptor mRNA, and the expression of smal intestine CaBp-D9K mRNA were increased in moderate-and high-dose flavonoids groups and vitamin D3 group. Results indicated that flavonoids from Cuscuta chinensis could significantly raise bone mineral density of femur, and 1,25(OH)2D3 levels of serum and kidney and the expression of lumbar vertebra vitamin D receptor mRNA and the expression of smal intestine CaBp-D9K mRNA, accelerate intestinal calcium absorption and osteoblast activity, and reinforce quality of the bone.