CAJ | 학술논문

目的观察吗啡条件性位置偏爱(conditioned place preference,CPP)大鼠纹状体中多巴胺(DA)递质和多巴胺D2受体的同步变化,从神经递质和受体分子水平探讨通过纹状体多巴胺能系统的阿片类精神依赖机制.方法吗啡剂量递增注射10d,建立大鼠吗啡CPP模型,生理盐水对照组和吗啡模型组各取10只断头处死取脑纹状体,采用高效液相色谱法测定其多巴胺含量；另各取6只采用免疫组化检测纹状体中D2受体的表达.结果与生理盐水对照组比较[DA:(5.23±1.01);D2受体:(0.08±0.02)],模型组大鼠纹状体多巴胺递质含量增加(7.63±0.98)；多巴胺D2受体平均吸光度值下调(0.06±0.02),均差异有统计学意义(P＜0.01).结论纹状体多巴胺递质含量上升和D2受体表达水平下调的同步变化在一定程度上参与了吗啡精神依赖的形成.
목적 관찰마배조건성위치편애(conditioned place preference,CPP)대서문상체중다파알(DA)체질화다파알D2수체적동보변화,종신경체질화수체분자수평탐토통과문상체다파알능계통적아편류정신의뢰궤제.방법 마배제량체증주사10d,건립대서마배CPP모형,생리염수대조조화마배모형조각취10지단두처사취뇌문상체,채용고효액상색보법측정기다파알함량；령각취6지채용면역조화검측문상체중D2수체적표체.결과 여생리염수대조조비교[DA:(5.23±1.01);D2수체:(0.08±0.02)],모형조대서문상체다파알체질함량증가(7.63±0.98)；다파알D2수체평균흡광도치하조(0.06±0.02),균차이유통계학의의(P＜0.01).결론 문상체다파알체질함량상승화D2수체표체수평하조적동보변화재일정정도상삼여료마배정신의뢰적형성.
Objective To observe the level of dopamine and its receptor in striatum from conditioned place preference (CPP) of rats administrated of morphine.And explore the mechanism of opioid-psychic dependence involving the levels of neurotransmitter and receptor.Methods CPP model was validated in morphine-dependence rats for 10 days.Striatum samples were harvested from two separate groups involving the saline group and morphine treated group (n =10 per experiment).The DA contents in striatum were detected in rats with colorimetry.Immunohistochemistry was applied to detect the differential expression levels of dopamine receptor 2 (DRD2)in samples.Results Compared with the saline group,higher content of neurotransmitter dopamine was observed in the morphine dependent rats (7.63 ±0.98 vs 5.23 ± 1.01,P＜0.01),while the expression level of DRD2 was down regulated (0.06 ± 0.02 vs 0.08 ± 0.02,P ＜ 0.01).Conclusion The increased expression of neurotransmitter dopamine and the decreased of DRD2 may be contributed to morphine-induced psychological dependence in morphine dependent rats.