中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
12期
1073-1076
,共4页
褪黑素%高血压%学习记忆%吻侧迁移流%嗅球
褪黑素%高血壓%學習記憶%吻側遷移流%嗅毬
퇴흑소%고혈압%학습기억%문측천이류%후구
Melatonin%Hypertension%Spatial memory%Rostral migratory stream%Olfactory bulb
目的 探讨褪黑素对高血压大鼠学习记忆及吻侧迁移流(RMS)和嗅球神经发生的影响.方法 建立肾性高血压大鼠模型,将大鼠分为高血压组、假手术组、褪黑素组、正常对照组,采用Morris水迷宫、BrdU单标与BrdU+ NT或BrdU+ GFAP双标免疫组化方法,测定各组大鼠行为、神经干细胞增殖与分化的状态.结果 水迷宫实验:高血压组逃避潜伏期[(29.95 ±20.11)s]比正常对照组[(20.58±19.18)s]、假手术组[(22.11 ±20.28)s]、褪黑素组[(23.81±22.25)s]明显延长,差异具有统计学意义(P＜0.05)；在原平台象限的游泳时间,高血压组[(25.51±5.21)s]比正常对照组[(34.67 ±4.53)s]、假手术组[(33.56 ±6.12)s]、褪黑素组[(36.23 ±3.86)s]明显缩短,差异具有统计学意义(P＜0.05).RMS的神经干细胞增殖:高血压组的BrdU阳性细胞数[(34.08±6.49)个/切片]比正常对照组[(43.53±7.56)个/切片]、假手术组[(46.11±4.75)个/切片]、褪黑素组[(44.30±8.29)个/切片]明显下降,差异有统计学意义(P＜0.05).嗅球神经干细胞分化:高血压组的BrdU+ GFAP阳性细胞数[(2.45 ±1.32)个/切片]分别比正常对照组[(1.01±0.78)个/切片]、假手术组[(0.68 ±0.37)个/切片]、褪黑素组[(1.35 ±0.88)个/切片]明显升高,差异具有统计学意义(P＜0.05)；高血压组的BrdU+NT阳性细胞数[(1.15±0.81)个/切片]分别比正常对照组[(1.89 ±0.98)个/切片]、假手术组[(1.63±1.01)个/切片]、褪黑素组[(2.30±1.22)个/切片]明显下降,差异具有统计学意义(P＜0.05).结论 褪黑素可能通过逆转神经发生障碍的方式来改善高血压诱发的学习记忆异常.
目的 探討褪黑素對高血壓大鼠學習記憶及吻側遷移流(RMS)和嗅毬神經髮生的影響.方法 建立腎性高血壓大鼠模型,將大鼠分為高血壓組、假手術組、褪黑素組、正常對照組,採用Morris水迷宮、BrdU單標與BrdU+ NT或BrdU+ GFAP雙標免疫組化方法,測定各組大鼠行為、神經榦細胞增殖與分化的狀態.結果 水迷宮實驗:高血壓組逃避潛伏期[(29.95 ±20.11)s]比正常對照組[(20.58±19.18)s]、假手術組[(22.11 ±20.28)s]、褪黑素組[(23.81±22.25)s]明顯延長,差異具有統計學意義(P＜0.05)；在原平檯象限的遊泳時間,高血壓組[(25.51±5.21)s]比正常對照組[(34.67 ±4.53)s]、假手術組[(33.56 ±6.12)s]、褪黑素組[(36.23 ±3.86)s]明顯縮短,差異具有統計學意義(P＜0.05).RMS的神經榦細胞增殖:高血壓組的BrdU暘性細胞數[(34.08±6.49)箇/切片]比正常對照組[(43.53±7.56)箇/切片]、假手術組[(46.11±4.75)箇/切片]、褪黑素組[(44.30±8.29)箇/切片]明顯下降,差異有統計學意義(P＜0.05).嗅毬神經榦細胞分化:高血壓組的BrdU+ GFAP暘性細胞數[(2.45 ±1.32)箇/切片]分彆比正常對照組[(1.01±0.78)箇/切片]、假手術組[(0.68 ±0.37)箇/切片]、褪黑素組[(1.35 ±0.88)箇/切片]明顯升高,差異具有統計學意義(P＜0.05)；高血壓組的BrdU+NT暘性細胞數[(1.15±0.81)箇/切片]分彆比正常對照組[(1.89 ±0.98)箇/切片]、假手術組[(1.63±1.01)箇/切片]、褪黑素組[(2.30±1.22)箇/切片]明顯下降,差異具有統計學意義(P＜0.05).結論 褪黑素可能通過逆轉神經髮生障礙的方式來改善高血壓誘髮的學習記憶異常.
목적 탐토퇴흑소대고혈압대서학습기억급문측천이류(RMS)화후구신경발생적영향.방법 건립신성고혈압대서모형,장대서분위고혈압조、가수술조、퇴흑소조、정상대조조,채용Morris수미궁、BrdU단표여BrdU+ NT혹BrdU+ GFAP쌍표면역조화방법,측정각조대서행위、신경간세포증식여분화적상태.결과 수미궁실험:고혈압조도피잠복기[(29.95 ±20.11)s]비정상대조조[(20.58±19.18)s]、가수술조[(22.11 ±20.28)s]、퇴흑소조[(23.81±22.25)s]명현연장,차이구유통계학의의(P＜0.05)；재원평태상한적유영시간,고혈압조[(25.51±5.21)s]비정상대조조[(34.67 ±4.53)s]、가수술조[(33.56 ±6.12)s]、퇴흑소조[(36.23 ±3.86)s]명현축단,차이구유통계학의의(P＜0.05).RMS적신경간세포증식:고혈압조적BrdU양성세포수[(34.08±6.49)개/절편]비정상대조조[(43.53±7.56)개/절편]、가수술조[(46.11±4.75)개/절편]、퇴흑소조[(44.30±8.29)개/절편]명현하강,차이유통계학의의(P＜0.05).후구신경간세포분화:고혈압조적BrdU+ GFAP양성세포수[(2.45 ±1.32)개/절편]분별비정상대조조[(1.01±0.78)개/절편]、가수술조[(0.68 ±0.37)개/절편]、퇴흑소조[(1.35 ±0.88)개/절편]명현승고,차이구유통계학의의(P＜0.05)；고혈압조적BrdU+NT양성세포수[(1.15±0.81)개/절편]분별비정상대조조[(1.89 ±0.98)개/절편]、가수술조[(1.63±1.01)개/절편]、퇴흑소조[(2.30±1.22)개/절편]명현하강,차이구유통계학의의(P＜0.05).결론 퇴흑소가능통과역전신경발생장애적방식래개선고혈압유발적학습기억이상.
Objective To investigate the possible abnormal changes of adult neurogenesis in the rostral migratory stream(RMS),in the olfactory bulb and in learning or memory of renovascular hypertensive rat,and to evaluate the possible efficacies produced by intervention of exogenous melatonin.Methods Renovascular hypertensive rat model was established by clamping bilateral renal arteries.Rats were randomly divided into four groups with 10 rats per group:hypertensive,sham-operated,melatonin-treated and normal groups.Morris water maze was used to detect the abilities of learning and memory,and immunohistochemistry for bromodeoxyuridine (BrdU),bromodeoxyuridine plus glial fibrillary acidic protein (BrdU + GFAP) and bromodeoxyuridine plus neurofilament (BrdU + NT) was utilized to examine the changes of the adult neurogenesis in RMS and in the olfactory bulb of each rat.Results The escape latency of hypertensive group ((29.95 ± 20.11) s) recorded in Morris water maze test was the longest in a significant comparison to those of normal group((20.58 ± 19.18)s),sham-operated group ((22.11 ± 20.28) s) and melatonin-treated group ((23.81 ± 22.25) s) (P ＜ 0.05) ; while the time spent by rats during swimming in the quadrant with being originally put platform of hypertensive ((25.51 ± 5.21) s) was the shortest by significant comparison to those of normal((34.67 ± 4.53) s),sham-operated ((33.56 ± 6.12) s) and melatonin-treated group ((36.23 ± 3.86)s) (P ＜ 0.05).Proliferation of neural stem cells (cells/field):the number of BrdU immunoreactive (BrdU-IR) cells of rats in RMS of hypertensive group(34.08 ±6.49) was the smallest,significantly compared with those of normal(43.53 ± 7.56),sham-operated (46.11 ± 4.75) and melatonintreated group (44.30 ± 8.29),(P ＜ 0.05).Differentiation of neural stem cells (cells/slice):the number of BrdU + GFAP-IR cells in olfactory bulb of hypertensive group (2.45 ± 1.32) was largest,significantly compared with those of normal (1.01 ± 0.78),sham-operated (0.68 ± 0.37) and melatonin-treated group (1.35 ± 0.88) (P ＜0.05) ; while the number of BrdU + NF-IR cells in olfactory bulb of hypertensive group (1.15 ± 0.81) was the smallest,significantly compared with those of normal (1.89 ± 0.98),sham-operated(1.63 ± 1.01) and melatonin-treated group (2.30 ± 1.22) (P ＜ 0.05).Conclusion Melatonin can ameliorate the abnormal cognitive behaviors induced by hypertension via a mechanism of reversing the progression of neurogenesis disorders both in RMS and in olfactory bulb,indicating that melatonin may prevent the development of dementia triggered by hypertension.
