中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
2期
185-188
,共4页
唐秀能%韦红棉%陆翠林%李建哲%唐云峡
唐秀能%韋紅棉%陸翠林%李建哲%唐雲峽
당수능%위홍면%륙취림%리건철%당운협
藿香蓟%乙醇提取物%抗炎作用%机制
藿香薊%乙醇提取物%抗炎作用%機製
곽향계%을순제취물%항염작용%궤제
Ageratum conyzoides. L.%Ethanol extracts%Anti-inflammation%Mechanism
目的：：探讨桂产藿香蓟乙醇提取物的抗炎作用及其作用机制。方法：采用二甲苯诱导小鼠耳廓肿胀,观察桂产藿香蓟乙醇提取物对二甲苯诱导小鼠耳廓肿胀的影响；采用角叉菜胶致小鼠足肿胀法,观察桂产藿香蓟乙醇提取物对角叉菜胶致小鼠足肿胀的影响,并分别测定小鼠炎性组织中丙二醛( MDA)、前列腺素E2( PGE2)和超氧化物歧化酶( SOD)的活性；采用角叉菜胶致大鼠足肿胀法,观察桂产藿香蓟乙醇提取物对角叉菜胶诱导大鼠足肿胀的影响,同时测定大鼠血清肿瘤坏死因子(TNF-α)、白介素-1β(IL-1β)和白介素-6(IL-6)的含量。结果：与空白对照组比较,桂产藿香蓟乙醇提取物能显著抑制小鼠耳廓肿胀及足肿胀(P <0.05或0.01),其中高、中、低剂量组(6.0,3.0,1.5 g·kg-1)耳廓肿胀和足肿胀抑制率分别为29.24%,16.42%,11.21%和28.66%,18.79%,13.13%,并能降低小鼠炎足中炎性组织PGE2、MDA含量,提高其SOD活力( P<0.05或0.01)；显著抑制大鼠的足肿胀(P<0.05或0.01),其中在3 h时高、中、低剂量组(6.0,3.0,1.5 g·kg-1)的足肿胀抑制率分别为43.69%,36.01%,23.29%,并能显著降低肿胀足大鼠血清TNF-α、IL-1β和IL-6的含量(P<0.05或0.01)。结论：桂产藿香蓟乙醇提取物抗炎作用显著,其机制可能与清除氧自由基、减少炎症因子和致炎细胞因子的释放有关。
目的：：探討桂產藿香薊乙醇提取物的抗炎作用及其作用機製。方法：採用二甲苯誘導小鼠耳廓腫脹,觀察桂產藿香薊乙醇提取物對二甲苯誘導小鼠耳廓腫脹的影響；採用角扠菜膠緻小鼠足腫脹法,觀察桂產藿香薊乙醇提取物對角扠菜膠緻小鼠足腫脹的影響,併分彆測定小鼠炎性組織中丙二醛( MDA)、前列腺素E2( PGE2)和超氧化物歧化酶( SOD)的活性；採用角扠菜膠緻大鼠足腫脹法,觀察桂產藿香薊乙醇提取物對角扠菜膠誘導大鼠足腫脹的影響,同時測定大鼠血清腫瘤壞死因子(TNF-α)、白介素-1β(IL-1β)和白介素-6(IL-6)的含量。結果：與空白對照組比較,桂產藿香薊乙醇提取物能顯著抑製小鼠耳廓腫脹及足腫脹(P <0.05或0.01),其中高、中、低劑量組(6.0,3.0,1.5 g·kg-1)耳廓腫脹和足腫脹抑製率分彆為29.24%,16.42%,11.21%和28.66%,18.79%,13.13%,併能降低小鼠炎足中炎性組織PGE2、MDA含量,提高其SOD活力( P<0.05或0.01)；顯著抑製大鼠的足腫脹(P<0.05或0.01),其中在3 h時高、中、低劑量組(6.0,3.0,1.5 g·kg-1)的足腫脹抑製率分彆為43.69%,36.01%,23.29%,併能顯著降低腫脹足大鼠血清TNF-α、IL-1β和IL-6的含量(P<0.05或0.01)。結論：桂產藿香薊乙醇提取物抗炎作用顯著,其機製可能與清除氧自由基、減少炎癥因子和緻炎細胞因子的釋放有關。
목적：：탐토계산곽향계을순제취물적항염작용급기작용궤제。방법：채용이갑분유도소서이곽종창,관찰계산곽향계을순제취물대이갑분유도소서이곽종창적영향；채용각차채효치소서족종창법,관찰계산곽향계을순제취물대각차채효치소서족종창적영향,병분별측정소서염성조직중병이철( MDA)、전렬선소E2( PGE2)화초양화물기화매( SOD)적활성；채용각차채효치대서족종창법,관찰계산곽향계을순제취물대각차채효유도대서족종창적영향,동시측정대서혈청종류배사인자(TNF-α)、백개소-1β(IL-1β)화백개소-6(IL-6)적함량。결과：여공백대조조비교,계산곽향계을순제취물능현저억제소서이곽종창급족종창(P <0.05혹0.01),기중고、중、저제량조(6.0,3.0,1.5 g·kg-1)이곽종창화족종창억제솔분별위29.24%,16.42%,11.21%화28.66%,18.79%,13.13%,병능강저소서염족중염성조직PGE2、MDA함량,제고기SOD활력( P<0.05혹0.01)；현저억제대서적족종창(P<0.05혹0.01),기중재3 h시고、중、저제량조(6.0,3.0,1.5 g·kg-1)적족종창억제솔분별위43.69%,36.01%,23.29%,병능현저강저종창족대서혈청TNF-α、IL-1β화IL-6적함량(P<0.05혹0.01)。결론：계산곽향계을순제취물항염작용현저,기궤제가능여청제양자유기、감소염증인자화치염세포인자적석방유관。
Objective:To explore the anti-inflammatory effects and the underlying mechanisms of ethanol extract of Ageratum cony-zoides. L. from Guangxi. Methods:The auricle edema model was induced by dimethylbenzene in the mice and the paw edema model was induced by carrageenan respectively in the mice and rats to study the anti-inflammatory effects of ethanol extract of Ageratum cony-zoides. L. from Guangxi. The content of malondiadehyde (MDA) and proateglandin E2 (PGE2), and the activity of superoxide dis-mutase( SOD) in the mouse edema paw was measured. The contents of tumour necrosisfactor-α ( TNF-α) , interleukin-1β ( IL-1β) and interleukin-6(IL-6) in the rat serum were detected as well. Results:Compared with the model control group, the ethanol extracts of Ageratum conyzoides. L. from Guangxi could remarkably inhibit auricle edema in the mice and paw edema in the mice and rats( P<0. 05 or P<0. 01), the inhibition ratio for high, medium and low dosage group(6. 0, 3. 0, 1. 5 g·kg-1)was 29. 24%,16. 42% and 11. 21% in the auricle edema mice and 28. 66%,18. 79% and 13. 13% in the paw edema mice , respectively. It could remarkably re-duce MDA and PGE2 content and enhance the activity of SOD in the mouse inflammatory tissue(P<0. 05 or P<0. 01). In the paw e-dema rats, the inhibition ratio for high, medium and low dosage group(4. 5,2. 3, 1. 2 g·kg-1)was 43. 69%, 36. 01% and 23. 29%at the 3rd h, respectively , and it also could remarkably reduce serum TNF-α, IL-1βand IL-6 content(P<0. 05 or P<0. 01). Con-clusion:The ethanol extracts of Ageratum conyzoides. L. from Guangxi show significantly anti-inflammatory effects, and the mecha-nisms may be related to the ability of scavenging oxygen free radicals and reducing the release of inflammatory cytokines and proinflam-matory cytokines.
