中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
27期
4395-4400
,共6页
解力%张晓刚%黄吕%李永林%汤小曼%张辉
解力%張曉剛%黃呂%李永林%湯小曼%張輝
해력%장효강%황려%리영림%탕소만%장휘
实验动物%组织构建%移植%自体心包膜移植%带瓣移植%心肌梗死%室性早搏%细胞凋亡
實驗動物%組織構建%移植%自體心包膜移植%帶瓣移植%心肌梗死%室性早搏%細胞凋亡
실험동물%조직구건%이식%자체심포막이식%대판이식%심기경사%실성조박%세포조망
transplants%pericardium%heart valves%myocardial infarction%ventricular premature complexes
背景：自体心包膜移植治疗已广泛应用于临床，主要涉及心血管修补重建、眼表疾病的治疗等领域；但其对心脏本身在缺血损伤下的保护作用却研究甚少。探索自体心包膜带瓣移植的安全性及其对缺血损伤后心肌的保护作用具有重要意义。<br> 目的：观察心脏接受自体心包膜移植后对心电活动的影响及其对缺血心肌的保护作用。<br> 方法：以荣昌肉猪和SD大鼠为研究对象，2种动物分别分为3组：移植组、心肌梗死组及心肌梗死+移植组，心肌梗死组及心肌梗死+移植组动物结扎冠状动脉前降支建立心肌梗死模型，移植组及心肌梗死+移植组动物以自体心包膜组织带瓣移植建立移植模型。<br> 结果与结论：①猪心电图监测室上性早搏常见；移植组室性早搏偶见，监测过程中未见室性心动过速和室性颤动。与心肌梗死组相比，心肌梗死+移植组室性早搏减少，超声心动图检查示心功能改善(P <0.05)。②移植组大鼠心电图监测未见室性颤动；心肌梗死组、心肌梗死+移植组均见非致死性室性颤动。与心肌梗死组相比，心肌梗死+移植组心功能改善，心肌凋亡指数降低，Bcl-2蛋白表达升高，Caspase-3蛋白表达降低(P <0.05)。提示自体心包膜带瓣移植未诱发恶性室性心律失常，其安全性相对较高；并在一定程度上减少缺血损伤后室性早搏个数、促进心功能恢复，其可能与抑制缺血区域细胞凋亡有关。
揹景：自體心包膜移植治療已廣汎應用于臨床，主要涉及心血管脩補重建、眼錶疾病的治療等領域；但其對心髒本身在缺血損傷下的保護作用卻研究甚少。探索自體心包膜帶瓣移植的安全性及其對缺血損傷後心肌的保護作用具有重要意義。<br> 目的：觀察心髒接受自體心包膜移植後對心電活動的影響及其對缺血心肌的保護作用。<br> 方法：以榮昌肉豬和SD大鼠為研究對象，2種動物分彆分為3組：移植組、心肌梗死組及心肌梗死+移植組，心肌梗死組及心肌梗死+移植組動物結扎冠狀動脈前降支建立心肌梗死模型，移植組及心肌梗死+移植組動物以自體心包膜組織帶瓣移植建立移植模型。<br> 結果與結論：①豬心電圖鑑測室上性早搏常見；移植組室性早搏偶見，鑑測過程中未見室性心動過速和室性顫動。與心肌梗死組相比，心肌梗死+移植組室性早搏減少，超聲心動圖檢查示心功能改善(P <0.05)。②移植組大鼠心電圖鑑測未見室性顫動；心肌梗死組、心肌梗死+移植組均見非緻死性室性顫動。與心肌梗死組相比，心肌梗死+移植組心功能改善，心肌凋亡指數降低，Bcl-2蛋白錶達升高，Caspase-3蛋白錶達降低(P <0.05)。提示自體心包膜帶瓣移植未誘髮噁性室性心律失常，其安全性相對較高；併在一定程度上減少缺血損傷後室性早搏箇數、促進心功能恢複，其可能與抑製缺血區域細胞凋亡有關。
배경：자체심포막이식치료이엄범응용우림상，주요섭급심혈관수보중건、안표질병적치료등영역；단기대심장본신재결혈손상하적보호작용각연구심소。탐색자체심포막대판이식적안전성급기대결혈손상후심기적보호작용구유중요의의。<br> 목적：관찰심장접수자체심포막이식후대심전활동적영향급기대결혈심기적보호작용。<br> 방법：이영창육저화SD대서위연구대상，2충동물분별분위3조：이식조、심기경사조급심기경사+이식조，심기경사조급심기경사+이식조동물결찰관상동맥전강지건립심기경사모형，이식조급심기경사+이식조동물이자체심포막조직대판이식건립이식모형。<br> 결과여결론：①저심전도감측실상성조박상견；이식조실성조박우견，감측과정중미견실성심동과속화실성전동。여심기경사조상비，심기경사+이식조실성조박감소，초성심동도검사시심공능개선(P <0.05)。②이식조대서심전도감측미견실성전동；심기경사조、심기경사+이식조균견비치사성실성전동。여심기경사조상비，심기경사+이식조심공능개선，심기조망지수강저，Bcl-2단백표체승고，Caspase-3단백표체강저(P <0.05)。제시자체심포막대판이식미유발악성실성심률실상，기안전성상대교고；병재일정정도상감소결혈손상후실성조박개수、촉진심공능회복，기가능여억제결혈구역세포조망유관。
BACKGROUND:The treatment of autologous pericardium transplantation has been widely applied in clinics, mainly involving cardiovascular repair and reconstruction, the treatment of ocular surface disease. The study addressing protection effects of autologous pericardium transplantation on the heart with ischemia injury is rarely reported. The investigations on the safety and protection effects of autologous pericardial transplantation on the heart with ischemia injury are of important significance. <br> OBJECTIVE:To explore effect of autologous pericardial transplantation on cardiac electrical activity and the protective effects on myocardial ischemia. <br> METHODS:Rongchang pork pigs and Sprague-Dawley rats were randomly divided into three groups:autologous pericardium transplantation, myocardial ischemia, and myocardial ischemia+autologous pericardium transplantation. The model of myocardial ischemia was established by ligation of the left anterior descending coronary artery in the groups of myocardial ischemia and myocardial ischemia+autologous pericardium transplantation. The model of transplantation was established by autologous pericardium transplant with flap in the groups of autologous pericardium transplantation and myocardial ischemia+autologous pericardium transplantation. <br> RESULTS AND CONCLUSION:Porcine electrocardiogram monitoring results showed that, superventricular premature beat was frequently observed in each group of pigs;the ventricular premature beat was occasional observed in autologous pericardium transplantation group, ventricular tachycardia and ventricular fibril ation did not appear. Compared with myocardial ischemia group, the ventricular premature beat decreased and the heart function was improved in myocardial ischemia+autologous pericardium transplantation group (P<0.05). Rat electrocardiogram monitoring results showed that, the ventricular fibril ation did not appear in autologous pericardium transplantation group, the lethal ventricular fibril ation did not appear in myocardial ischemia and myocardial ischemia+autologous pericardium transplantation groups. Compared with myocardial ischemia group, the heart function was improved, the apoptosis index decreased, the expressions of Bcl-2 protein increased, the expressions of Caspase-3 protein decreased in myocardial ischemia+autologous pericardium transplantation group (P<0.05). The autologous pericardium transplantation with flap cannot induce malignant ventricular arrhythmia and is relatively safe;the ventricular premature beat is reduced, the cardiac function is improved, which is possibly related to the inhibition of apoptosis in myocardial ischemic area.
