中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2014年
6期
361-364
,共4页
时春艳%赵扬玉%范玲%杨磊%杨慧霞%孙丽颖%曲首辉%邹丽颖%李诗兰%吴秉铨%姚晨
時春豔%趙颺玉%範玲%楊磊%楊慧霞%孫麗穎%麯首輝%鄒麗穎%李詩蘭%吳秉銓%姚晨
시춘염%조양옥%범령%양뢰%양혜하%손려영%곡수휘%추려영%리시란%오병전%요신
链球菌,无乳%妊娠末期%实时聚合酶链反应%多中心研究
鏈毬菌,無乳%妊娠末期%實時聚閤酶鏈反應%多中心研究
련구균,무유%임신말기%실시취합매련반응%다중심연구
Streptococcus agalactiae%Pregnancy trimester,third%Realtime polymerase chain reaction%Multicenter study
目的探讨实时聚合酶链反应（polymerase chain reaction，PCR）技术检测妊娠晚期孕妇B族溶血性链球菌（group B Streptococcus，GBS）的准确性。方法本研究为多中心研究。选择2009年3月1日至12月31日在北京大学第一医院妇产科、首都医科大学附属北京妇产医院产科和北京大学第三医院妇产科产前保健的妊娠35~37周孕妇，取阴道下1/3分泌物及肛周分泌物，采用常规细菌培养法及实时PCR方法进行GBS检测。采用基因测序作为矫正方法，分析实时PCR方法检测GBS的敏感性和特异性。结果（1）3家医院共收集1395份标本，细菌培养法检测GBS阳性40例（2.9%），实时PCR方法检测GBS阳性114例（8.2%）。（2）仅实时PCR方法检测GBS阳性者77例，采用事先设计好的第2对引物扩增后进行测序，检测鉴定为GBS序列的共66例，11例为非GBS。（3）以细菌培养法加测序法校正作为金标准，实时PCR方法检测GBS的敏感性为97.2%（103/106），特异性为99.1%（1278/1289）。常规细菌培养法漏诊率62.3%（66/106）。（4）细菌培养法加测序法校正3家医院孕妇妊娠晚期GBS携带率为7.6%（106/1395）。结论实时PCR方法检测GBS具有较高的敏感性和特异性，有望成为妊娠晚期常规检测GBS的方法。
目的探討實時聚閤酶鏈反應（polymerase chain reaction，PCR）技術檢測妊娠晚期孕婦B族溶血性鏈毬菌（group B Streptococcus，GBS）的準確性。方法本研究為多中心研究。選擇2009年3月1日至12月31日在北京大學第一醫院婦產科、首都醫科大學附屬北京婦產醫院產科和北京大學第三醫院婦產科產前保健的妊娠35~37週孕婦，取陰道下1/3分泌物及肛週分泌物，採用常規細菌培養法及實時PCR方法進行GBS檢測。採用基因測序作為矯正方法，分析實時PCR方法檢測GBS的敏感性和特異性。結果（1）3傢醫院共收集1395份標本，細菌培養法檢測GBS暘性40例（2.9%），實時PCR方法檢測GBS暘性114例（8.2%）。（2）僅實時PCR方法檢測GBS暘性者77例，採用事先設計好的第2對引物擴增後進行測序，檢測鑒定為GBS序列的共66例，11例為非GBS。（3）以細菌培養法加測序法校正作為金標準，實時PCR方法檢測GBS的敏感性為97.2%（103/106），特異性為99.1%（1278/1289）。常規細菌培養法漏診率62.3%（66/106）。（4）細菌培養法加測序法校正3傢醫院孕婦妊娠晚期GBS攜帶率為7.6%（106/1395）。結論實時PCR方法檢測GBS具有較高的敏感性和特異性，有望成為妊娠晚期常規檢測GBS的方法。
목적탐토실시취합매련반응（polymerase chain reaction，PCR）기술검측임신만기잉부B족용혈성련구균（group B Streptococcus，GBS）적준학성。방법본연구위다중심연구。선택2009년3월1일지12월31일재북경대학제일의원부산과、수도의과대학부속북경부산의원산과화북경대학제삼의원부산과산전보건적임신35~37주잉부，취음도하1/3분비물급항주분비물，채용상규세균배양법급실시PCR방법진행GBS검측。채용기인측서작위교정방법，분석실시PCR방법검측GBS적민감성화특이성。결과（1）3가의원공수집1395빈표본，세균배양법검측GBS양성40례（2.9%），실시PCR방법검측GBS양성114례（8.2%）。（2）부실시PCR방법검측GBS양성자77례，채용사선설계호적제2대인물확증후진행측서，검측감정위GBS서렬적공66례，11례위비GBS。（3）이세균배양법가측서법교정작위금표준，실시PCR방법검측GBS적민감성위97.2%（103/106），특이성위99.1%（1278/1289）。상규세균배양법루진솔62.3%（66/106）。（4）세균배양법가측서법교정3가의원잉부임신만기GBS휴대솔위7.6%（106/1395）。결론실시PCR방법검측GBS구유교고적민감성화특이성，유망성위임신만기상규검측GBS적방법。
Objective To evaluate the accuracy of realtime polymerase chain reaction (PCR) assay in the detection of group B Streptococcus (GBS) in pregnant women. Methods Samples were collected from 1 395 women at 35-37 weeks of gestation from March 1 to December 31, 2009 at three hospitals in Beijing. Samples were obtained from the lower one third vaginal wall and perianal area and tested for GBS using standard culture and PCR. Standard culture and gene analysis for GBS were applied as the gold standard, and the sensitivity and specificity of the rapid assay were determined. Results Of the 1 395 women qualified for PCR testing, 40(2.9%) were identified as GBS positive on the basis of the results of specimen culture, compared to 114 (8.2%) on the basis of PCR assay. The culture was negative and the PCR positive in 77 patients. The results which were not in agreement using the two tests were evaluated by the gene analysis for GBS. Among the 77 samples which were GBS positive by PCR, 66 samples were determined as GBS positive by gene analysis. The sensitivity of the PCR assay was 97.2%(103/106) and specificity was 99.1%(1 278/1 289), the maternal GBS colonization was 7.6%(106/1 395). Conclusions Realtime PCR assay allows rapid and reliable detection of GBS in last trimester with high sensitivity and specificity.
