CAJ | 학술논문

目的：制定艾滋病口腔含漱液的质量标准。方法：采用薄层色谱法鉴别处方中黄连、苦参二味中药材,采用高效液相法对盐酸小檗碱进行了定量,采用色谱柱：WondaSil Superb C18(4.6×250mm,5μm),流动相：乙腈：0.033mol·L-1磷酸二氢钠水溶液(30：70),检测波长：346nm,流速：1.0ml?min-1。柱温：35℃。结果：薄层鉴别色谱斑点清晰,分离度好,阴性对照无干扰；盐酸小檗碱的线性范围为(4.82~60.2)μg?L-1(r=0.9999)。结论：定性定量分析方法简便、准确、专属性强,可控制艾滋病口腔含漱液的质量。
목적：제정애자병구강함수액적질량표준。방법：채용박층색보법감별처방중황련、고삼이미중약재,채용고효액상법대염산소벽감진행료정량,채용색보주：WondaSil Superb C18(4.6×250mm,5μm),류동상：을정：0.033mol·L-1린산이경납수용액(30：70),검측파장：346nm,류속：1.0ml?min-1。주온：35℃。결과：박층감별색보반점청석,분리도호,음성대조무간우；염산소벽감적선성범위위(4.82~60.2)μg?L-1(r=0.9999)。결론：정성정량분석방법간편、준학、전속성강,가공제애자병구강함수액적질량。
To establish the Love mouth gargle quality standards.Methods:Identification by TLC prescription berberine,Sophora two flavor herbs,using high performance liquid chromatography on a quantitative berberine hydrochloride,HPLC method for the quantitative berberine hydrochloride,using Column:WondaSil Superb C18 (4.6×250mm,5μm),mobile phase:acetonitrile:0.033mol?L-1:sodium dihydrogen phosphate solution (30:70 ), detection wavelength:346nm, flow rate:1.0ml?min-1.Column temperature:35℃.Results:The TLC spots were clear,the separation degree is good,no negative interference;linear range of berberine hydrochloride was (4.82~60.2)μg?L-1 (r=0.9999). Conclusion:The qualitative and quantitative analysis method is simple,accurate,specific,and can control the quality of love mouth gargle.