CAJ | 학술논문

目的：探讨CD8＋CD28-T细胞在子宫内膜异位症（ EMT）发病机制中的作用。方法收集EMT患者合并盆腔积液80例（Ⅰ期15例、Ⅱ期12例、Ⅲ期22例、Ⅳ期31例），采用流式细胞术检测其外周血及盆腔积液内CD8＋CD28-T细胞及其胞内细胞因子转化生长因子β1（TGF-β1）及白介素10（IL-10）比率，并选取同期年龄相似的20例健康体检女性志愿者为对照组。结果与健康对照组比较，Ⅰ～Ⅳ期EMT患者外周血CD8＋CD28-T细胞、TGF-β1及IL-10比率均明显升高，表现为Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，且两两比较差异均有统计学意义（ P ＜0.05）；EMT患者盆腔积液CD8＋CD28-T细胞、TGF-β1及IL-10的频率同样表现为Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，且以TGF-β1的组间差异最为明显（ P ＜0.05）。 CD8＋CD28-T细胞、TGF-β1及IL-10与分期呈正相关（ rs ＝0.791，0.753，0.726， P ＜0.05）。结论 CD8＋CD28-T细胞、TGF-β1及IL-10在EMT发病机制当中具有重要作用，三者可能是EMT的一个不利因素，并且与EMT的分期密切相关；细胞因子TGF-β1在EMT盆腔积液形成过程中起到重要作用。
목적：탐토CD8＋CD28-T세포재자궁내막이위증（ EMT）발병궤제중적작용。방법수집EMT환자합병분강적액80례（Ⅰ기15례、Ⅱ기12례、Ⅲ기22례、Ⅳ기31례），채용류식세포술검측기외주혈급분강적액내CD8＋CD28-T세포급기포내세포인자전화생장인자β1（TGF-β1）급백개소10（IL-10）비솔，병선취동기년령상사적20례건강체검녀성지원자위대조조。결과여건강대조조비교，Ⅰ～Ⅳ기EMT환자외주혈CD8＋CD28-T세포、TGF-β1급IL-10비솔균명현승고，표현위Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，차량량비교차이균유통계학의의（ P ＜0.05）；EMT환자분강적액CD8＋CD28-T세포、TGF-β1급IL-10적빈솔동양표현위Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，차이TGF-β1적조간차이최위명현（ P ＜0.05）。 CD8＋CD28-T세포、TGF-β1급IL-10여분기정정상관（ rs ＝0.791，0.753，0.726， P ＜0.05）。결론 CD8＋CD28-T세포、TGF-β1급IL-10재EMT발병궤제당중구유중요작용，삼자가능시EMT적일개불리인소，병차여EMT적분기밀절상관；세포인자TGF-β1재EMT분강적액형성과정중기도중요작용。
Objective To explore the expression and clinical significance of CD 8 +CD28 -T lymphocytes in the patients with endometriosis ( EMT) complicating with pelvic fluid .Methods Eighty patients with endometriosis complicating with pelvic fluid ( ca-ses of ⅠtoⅣwere 15, 12, 22, and 31, respectively) were enrolled.The peripheral blood as well as the pelvic fluid were collected and flow cytometry was applied to detect the frequencies of CD 8 +CD28 -T lymphocytes and their intracellular cytokines , including transforming growth factor β1 (TGF-β1) and interleukin 10 (IL-10).Twenty health women with the same range of age were enrolled as the control group .Results Compared with the control ones , the frequencies of peripheral blood CD 8 +CD28 -T lymphocytes , TGF-β1, and IL-10 of the EMT subjects were increased significantly (all P <0.05), which presented the order Ⅳ>Ⅲ>Ⅱ>Ⅰand the difference between every two of the Ⅰ~Ⅳgroups was significant (all P <0.05).Similarly, the data of CD8 +CD28 -T lymphocytes, TGF-β1, and IL-10 in pelvic fluid also presented as Ⅳ>Ⅲ>Ⅱ>Ⅰ.Moreover, the difference of pelvic fluid TGF-β1 between any two groups was more apparent compared with CD 8 +CD28 -T lymphocytes and IL-10 ( P <0.05 ) .Spearman correlation analysis showed that any one of CD8 +CD28 -T lymphocytes, TGF-β1, and IL-10 positively correlated with any stage of ⅠtoⅣ( rs =0.791, 0.753,0.726 and all P <0.05).Conclusions CD8 +CD28 -T lymphocytes, TGF-β1, and IL-10 were closely related to the stages and could be the negative roles in the pathogenesis of EMT .TGF-β1 played a vital role in the formation of pelvic fluid .