CAJ | 학술논문

目的：放射治疗（ radiotherapy ， RT）已成为治疗恶性肿瘤的主要手段之一，年轻女性患者经放疗后往往会导致卵巢功能减退甚至衰竭，如何减轻放疗所致的卵巢功能损伤至关重要。本研究探讨褪黑素（ melatonin， MT）对射线照射后大鼠卵巢功能的保护作用。方法30只雌性SD大鼠随机均分为对照组、RT组、RT＋MT（25 mg/kg）组、RT＋MT（50 mg/kg）组、RT＋MT（100 mg/kg）组，分别接受等渗盐水注射、200 cGy射线照射＋等渗盐水注射和200 cGy射线照射＋不同剂量的MT注射。射线照射两周后处死，采用酶联免疫吸附法测定各组大鼠血清卵泡刺激素（ serum follicle stimulating hormone ， FSH）和雌二醇（estradiol， E2）浓度，计数各组卵泡数量，并采用分光光度法测定卵巢组织半胱氨酸天冬氨酸蛋白酶3（Caspase3）活性。结果对照组、RT＋MT（100 mg/kg）组、RT＋MT（50 mg/kg）组、RT组的血E2水平依次降低，分别为（6．68±0．48、5．73±1．36、4．26±0．44、2．83±0．51）pmol/L；FSH水平依次升高，分别为（0．340±0．011、0．431±0．053、0．479±0．023、0．604±0．028）ng/mL；卵巢组织卵泡总数依次减少，分别为（21．67±1．97、18．00±2．28、15．50±1．05、11．50±2．43）；Caspase3活性依次增强，分别为（0．14±0．03、0．26±0．06、0．36±0．05、0．50±0．05）；除RT＋MT（50 mg/kg）组与RT＋MT（100 mg/kg）组FSH水平比较差异无统计学意义（P＞0．05）；其余各项指标组间比较差异均有统计学意义（P＜0．05）。结论 MT能减少射线对大鼠卵巢功能的损伤，其机制可能与MT抑制射线诱导的Caspase3激活有关。
목적：방사치료（ radiotherapy ， RT）이성위치료악성종류적주요수단지일，년경녀성환자경방료후왕왕회도치란소공능감퇴심지쇠갈，여하감경방료소치적란소공능손상지관중요。본연구탐토퇴흑소（ melatonin， MT）대사선조사후대서란소공능적보호작용。방법30지자성SD대서수궤균분위대조조、RT조、RT＋MT（25 mg/kg）조、RT＋MT（50 mg/kg）조、RT＋MT（100 mg/kg）조，분별접수등삼염수주사、200 cGy사선조사＋등삼염수주사화200 cGy사선조사＋불동제량적MT주사。사선조사량주후처사，채용매련면역흡부법측정각조대서혈청란포자격소（ serum follicle stimulating hormone ， FSH）화자이순（estradiol， E2）농도，계수각조란포수량，병채용분광광도법측정란소조직반광안산천동안산단백매3（Caspase3）활성。결과대조조、RT＋MT（100 mg/kg）조、RT＋MT（50 mg/kg）조、RT조적혈E2수평의차강저，분별위（6．68±0．48、5．73±1．36、4．26±0．44、2．83±0．51）pmol/L；FSH수평의차승고，분별위（0．340±0．011、0．431±0．053、0．479±0．023、0．604±0．028）ng/mL；란소조직란포총수의차감소，분별위（21．67±1．97、18．00±2．28、15．50±1．05、11．50±2．43）；Caspase3활성의차증강，분별위（0．14±0．03、0．26±0．06、0．36±0．05、0．50±0．05）；제RT＋MT（50 mg/kg）조여RT＋MT（100 mg/kg）조FSH수평비교차이무통계학의의（P＞0．05）；기여각항지표조간비교차이균유통계학의의（P＜0．05）。결론 MT능감소사선대대서란소공능적손상，기궤제가능여MT억제사선유도적Caspase3격활유관。
Objective The radiotherapy is one of main treatments for patients with malignant tumor and leads to ovarian func-tion decreasing in young women .It is very important to protect ovarian function during the process of radiotherapy .The aim of this study is to investigate the effect of melatonin ( MT) agonist on radiotherapy-induced ovarian function damage in female rats . Methods Thirty SD rats were divided into five groups randomly , which received normal saline, 200 cGy radiotherapy+normal saline, 200 cGy radiotherapy+MT(25 mg/kg), 200 cGy radiotherapy+MT(50 mg/kg), and 200 cGy radiotherapy +MT(100 mg/kg), respectively.All rats were de-capitated two weeks after radiotherapy .Levels of serum follicle-stimulating hormone (FSH) and estradiol (E2) were measured by en-zyme-linked immune sorbent assay method;the number of the primordial follicles , the growth follicles and the mature follicle were ob-served;Caspase3 activity was assayed by spectrophotometry . Results In accordance with the normal control group , RT+MT (100 mg/kg) group, RT+MT (50 mg/kg) group,RT group , serum E2 levels decreased, respectively (6.68 ±0.48, 5.73 ±1.36, 4.26 ±0.44, 2.83 ±0.51)pmol/L;FSH levels increased, respectively (0.340 ±0.011, 0.431 ±0.053, 0.479 ±0.023, 0.604 ± 0.028)ng/mL ;the total number of follicles decreased ,respectively (21.67 ±1.97, 18.00 ±2.28, 15.50 ±1.05, 11.50 ±2.43);Caspase3 activity increased,respectively (0.14 ±0.03, 0.26 ±0.06, 0.36 ±0.05, 0.50 ±0.05).Except FSH had no significant difference between RT +MT(50 mg/kg)group and RT+MT(100 mg/kg) group(P>0.05), the rest indexes had significant difference between the two groups(P<0.05). Conclusion MT can diminish radiotherapy-induced ovarian damage in female rats, it may be related to the mechanism that MT inhibits the radiotherapy-induced activation of Caspase 3.