CAJ | 학술논문

木霉菌的许多种属都能够产生脂类物质，并且已经被用来研究脂类物质的生产。本研究主要探索光照和肌醇对木霉生物量积累和油脂量积累的作用。结果表明：与遮光处理组相比，正常光照培养条件更易于菌株成熟孢子的形成，并且正常光照培养也利于菌株L1生物量和油脂量的积累；肌醇的添加会促进菌株生物量的积累，但是对菌株L1油脂量的积累有一定的抑制作用，对菌株L2，相比无肌醇添加组，肌醇的添加在一定的培养时间上有利于油脂的形成。同时发现光照和肌醇能够影响木霉的脂类构成。
목매균적허다충속도능구산생지류물질，병차이경피용래연구지류물질적생산。본연구주요탐색광조화기순대목매생물량적루화유지량적루적작용。결과표명：여차광처리조상비，정상광조배양조건경역우균주성숙포자적형성，병차정상광조배양야리우균주L1생물량화유지량적적루；기순적첨가회촉진균주생물량적적루，단시대균주L1유지량적적루유일정적억제작용，대균주L2，상비무기순첨가조，기순적첨가재일정적배양시간상유리우유지적형성。동시발현광조화기순능구영향목매적지류구성。
The method of microbial biosynthesis in vivo in order for the preparation of Microbial Oil is a new path in development of oil resources .In order to optimize the fermentation conditions , first, the experiment use cell morphological and cytochemical methods ( Sudan Black staining ) for primary screening of two Trichoderma strains under different culture conditions .The results show that:under normal lighting culture conditions ,L1 strain grows by GMY40 liquid medium for the culture medium , pH 5.5, 28℃fermentation for 3 days, bringing about microbial fat content 15.11%, the biomass 9.62 g /L, oil yield 1.45 g /L .Coordinate expression is that Light and inositol can affect the lipid composition of Trichoderma by TLC analyze method .