CAJ | 학술논문

本试验旨在研究嗜酸乳杆菌活菌体和热灭活菌体在体外模拟条件下对 IPEC-J2细胞的黏附和黏附拮抗效应。用嗜酸乳杆菌活菌体和热灭活菌体处理 IPEC-J2细胞，染色后观察细菌对细胞的黏附性。另外，采用嗜酸乳杆菌活菌体和热灭活菌体，通过竞争抑制、排阻抑制和替换抑制3种不同的处理方式抑制大肠杆菌在 IPEC-J2细胞上的黏附，利用伊红美蓝琼脂培养基，检测嗜酸乳杆菌对大肠杆菌黏附的抑制率。结果表明：嗜酸乳杆菌活菌体和热灭活菌体都能很好地黏附到 IPEC-J2细胞上，每个细胞上黏附的热灭活菌数[（40±2）CFU]高于黏附的活菌数[（20±2）CFU]。2种状态的嗜酸乳杆菌都能很好地抑制大肠杆菌在 IPEC-J2细胞上的黏附。在竞争抑制和替换抑制试验中，活菌体的抑制率显著高于热灭活菌体（ P＜0．05）。然而，在排阻抑制试验中，热灭活菌体的抑制率显著高于活菌体（P＜0．05）。由此可知，嗜酸乳杆菌的活菌体和热灭活菌体都能很好地黏附到 IPEC-J2细胞上，抑制大肠杆菌对细胞的黏附和入侵，热灭活菌体可能主要通过竞争黏附位点来抑制大肠杆菌的黏附，而活菌体可能不只这一机制，还会在代谢过程中产生一些抑菌物质，抑制大肠杆菌的生长繁殖。
본시험지재연구기산유간균활균체화열멸활균체재체외모의조건하대 IPEC-J2세포적점부화점부길항효응。용기산유간균활균체화열멸활균체처리 IPEC-J2세포，염색후관찰세균대세포적점부성。령외，채용기산유간균활균체화열멸활균체，통과경쟁억제、배조억제화체환억제3충불동적처리방식억제대장간균재 IPEC-J2세포상적점부，이용이홍미람경지배양기，검측기산유간균대대장간균점부적억제솔。결과표명：기산유간균활균체화열멸활균체도능흔호지점부도 IPEC-J2세포상，매개세포상점부적열멸활균수[（40±2）CFU]고우점부적활균수[（20±2）CFU]。2충상태적기산유간균도능흔호지억제대장간균재 IPEC-J2세포상적점부。재경쟁억제화체환억제시험중，활균체적억제솔현저고우열멸활균체（ P＜0．05）。연이，재배조억제시험중，열멸활균체적억제솔현저고우활균체（P＜0．05）。유차가지，기산유간균적활균체화열멸활균체도능흔호지점부도 IPEC-J2세포상，억제대장간균대세포적점부화입침，열멸활균체가능주요통과경쟁점부위점래억제대장간균적점부，이활균체가능불지저일궤제，환회재대사과정중산생일사억균물질，억제대장간균적생장번식。
The adhesion and adhesion antagonistic effect of live and heat-killed Lactobacillus acidophilus on IPEC-J2 cells were studied in vitro. IPEC-J2 cells were treated with live and heat-killed Lactobacillus acidophi-lus,and then stained the cells and observed the adhesive property. Moreover,the inhibition ratio of live and heat-killed Lactobacillus acidophilus to inhibit the adhesion of Escherichia coli on IPEC-J2 cells through three treatments(competitive inhibition,exclusion inhibition and displacement inhibition)was assessed by eosin-methylene blue agar(EMB)culture medium. The results showed as follows:live and heat-killed Lactobacillus acidophilus all could adhere well to the IPEC-J2 cells. The number of heat-killed bacteria adhering to the per cell[(40±2)CFU]was higher than the number of live bacteria adhering to the per cell[(20±2)CFU]. The two forms of Lactobacillus acidophilus had a good ability to inhibit the Escherichia coli adhere to IPEC-J2 cells. In competitive and displacement inhibition experiments,live bacteria showed a significantly better inhibi-tion ratio than heat-killed bacteria(P<0.05),whereas,in exclusion inhibition experiment,heat-killed bacteria showed a significantly better inhibition ratio than live bacteria( P<0.05). In conclusion,live and heat-killed Lactobacillus acidophilus can adhere well to the IPEC-J2 cells,and are able to reduce the adhesion and inva-sion to IPEC-J2 cells by Escherichia coli. Heat-killed Lactobacillus acidophilus inhibits Escherichia coli adhe-sion mainly through competition for host cell-binding receptors. Apart from competition for host cell-binding re-ceptors,some antibacterial substances from live Lactobacillus acidophilus are able to inhibit the growth and re-production of Escherichia coli.