CAJ | 학술논문

以生物素含量分别为0．068、0．410、0．910、1．960、4．620和9．380 mg / kg DM 的6组等氮等能的实用饲料投喂初始体重为1．03 g 的凡纳滨对虾幼虾8周，以研究饲料中生物素含量对凡纳滨对虾幼虾生长性能、体成分及生化指标的影响，最终确定凡纳滨对虾幼虾对饲料中生物素的需求量。每组3个重复，每个重复40尾对虾。结果表明：凡纳滨对虾幼虾的增重率、特定生长率、饲料效率和蛋白质效率均以0．068 mg / kg DM 组最低，显著低于其他各组（ P＜0．05），其他各组间差异不显著（P＞0．05）。各组凡纳滨对虾幼虾的存活率、蛋白质沉积率和体成分均无显著差异（P＞0．05）。饲料中生物素含量对血清总蛋白、葡萄糖、总胆固醇、甘油三酯、丙二醛含量及超氧化物歧化酶活性无显著影响（P＞0．05）。以0．910 mg / kg DM 组的血清总抗氧化能力最高，显著高于0．068 mg / kg DM 组（P＜0．05），与其他组差异不显著（ P＞0．05）。以0．068 mg / kg DM组的血清过氧化氢酶活性最低，与0．410 mg / kg DM 组差异不显著（ P＞0．05），但显著低于其他各组（P＜0．05）。以4．620 mg / kg DM 组的血清乙酰辅酶 A 羧化酶活性最高，显著高于0．068、0．410和0．910 mg / kg DM 组（P＜0．05）。肝胰腺生物素含量随着饲料中生物素含量的升高而显著增高（P＜0．05）。以饲料效率和血清乙酰辅酶 A 羧化酶活性为评价指标，通过折线模型分析得出凡纳滨对虾幼虾对饲料中生物素的需求量分别为0．72和2．25 mg / kg DM。
이생물소함량분별위0．068、0．410、0．910、1．960、4．620화9．380 mg / kg DM 적6조등담등능적실용사료투위초시체중위1．03 g 적범납빈대하유하8주，이연구사료중생물소함량대범납빈대하유하생장성능、체성분급생화지표적영향，최종학정범납빈대하유하대사료중생물소적수구량。매조3개중복，매개중복40미대하。결과표명：범납빈대하유하적증중솔、특정생장솔、사료효솔화단백질효솔균이0．068 mg / kg DM 조최저，현저저우기타각조（ P＜0．05），기타각조간차이불현저（P＞0．05）。각조범납빈대하유하적존활솔、단백질침적솔화체성분균무현저차이（P＞0．05）。사료중생물소함량대혈청총단백、포도당、총담고순、감유삼지、병이철함량급초양화물기화매활성무현저영향（P＞0．05）。이0．910 mg / kg DM 조적혈청총항양화능력최고，현저고우0．068 mg / kg DM 조（P＜0．05），여기타조차이불현저（ P＞0．05）。이0．068 mg / kg DM조적혈청과양화경매활성최저，여0．410 mg / kg DM 조차이불현저（ P＞0．05），단현저저우기타각조（P＜0．05）。이4．620 mg / kg DM 조적혈청을선보매 A 최화매활성최고，현저고우0．068、0．410화0．910 mg / kg DM 조（P＜0．05）。간이선생물소함량수착사료중생물소함량적승고이현저증고（P＜0．05）。이사료효솔화혈청을선보매 A 최화매활성위평개지표，통과절선모형분석득출범납빈대하유하대사료중생물소적수구량분별위0．72화2．25 mg / kg DM。
An 8-week feeding trial was conducted to evaluate the effects of dietary biotin content on growth performance,body composition,biochemical indices of juvenile Pacific white shrimp( Litopenaeus van-namei),in order to estimate the dietary biotin requirement of juvenile Pacific white shrimp. Six isonitrogenous and isocaloric practical diets were formulated with graded dietary biotin contents of 0. 068,0. 410,0. 910, 1.960,4.620 and 9.380 mg / kg DM,respectively. Each diet was fed to one group of shrimps. Each group had 3 replicates and each replicate had 40 shrimps with the average body weight of 1.03 g. The results showed that weight gain rate( WGR),specific growth rate( SGR),feed efficiency( FE)and protein efficiency ratio (PER)in 0.068 mg / kg DM group were all the lowest,and significantly lower than those in other groups(P<0.05),but no significant differences were observed among other groups(P>0.05). No significant differences were observed in survival rate( SR),protein retention rate( PRR)and body composition among all groups (P>0.05). The contents of total protein(TP),glucose(GLU),total cholesterol( TC),triglyceride( TG) and malondialdehyde(MDA),and superoxide dismutase( SOD)activity in serum were not significantly af-fected by dietary biotin content( P > 0. 05). The highest serum total anti-oxidative capacity( T-AOC)was found in 0.910 mg / kg DM group,and significantly lower than that in 0.068 mg / kg DM group,but no signifi-cant differences were observed among other groups(P>0.05). The lowest serum catalase(CAT)activity was found in 0.068 mg / kg DM group,and significantly lower than that in other groups( P < 0.05)except for 0.410 mg / kg DM group. The highest serum acetyl CoA carboxylase ( ACC ) activity was found in 4.620 mg / kg DM group,and significantly higher than that in 0.680,0.410 and 0.910 mg / kg DM groups(P<0.05). Hepatopancreas biotin content was significantly increased with dietary biotin content increasing( P <0.05). The optimal dietary biotin requirements of juvenile Pacific white shrimp estimated using a broken-line model based on FE and serum ACC activity are 0.72 and 2.25 mg / kg,respectively.