浙江预防医学
浙江預防醫學
절강예방의학
ZHEJIANG JOURNAL OF PREVENTIVE MEDICINE
2014年
7期
664-666,670
,共4页
白色念珠菌%氟康唑%药物敏感性%RT-PCR%CDR基因
白色唸珠菌%氟康唑%藥物敏感性%RT-PCR%CDR基因
백색념주균%불강서%약물민감성%RT-PCR%CDR기인
Candida albicans%Fluconazole%Drug sensitivity%RT-PCR%Clinical drug resistance(CDR)gene
目的：调查白色念珠菌临床株对氟康唑的耐药情况，研究白色念珠菌临床耐药基因的表达与氟康唑耐药之间的关系。方法采用M27-A2微量肉汤稀释法，对某院微生物实验室2010年分离保存的221株来自就诊患者的痰、中断尿、粪便和咽拭子的白色念珠菌进行氟康唑MIC值测定。以白色念珠菌18S RNA为内参照，采用RT-PCR技术观察比较耐药株和敏感株组在不同氟康唑浓度下CDR1和CDR2基因的转录水平。结果221株白色念珠菌中，有57.47％（127株）对氟康唑敏感，10.86％（24株）剂量依赖敏感，31.67％（70株）耐药。不同药物浓度作用下，耐药株组CDR1和CDR2的表达量相对要高于敏感株组（P＜0.05）。结论白色念珠球菌对氟康唑耐药率较高，耐药株组CDR1和CDR2表达量高于敏感株组。
目的：調查白色唸珠菌臨床株對氟康唑的耐藥情況，研究白色唸珠菌臨床耐藥基因的錶達與氟康唑耐藥之間的關繫。方法採用M27-A2微量肉湯稀釋法，對某院微生物實驗室2010年分離保存的221株來自就診患者的痰、中斷尿、糞便和嚥拭子的白色唸珠菌進行氟康唑MIC值測定。以白色唸珠菌18S RNA為內參照，採用RT-PCR技術觀察比較耐藥株和敏感株組在不同氟康唑濃度下CDR1和CDR2基因的轉錄水平。結果221株白色唸珠菌中，有57.47％（127株）對氟康唑敏感，10.86％（24株）劑量依賴敏感，31.67％（70株）耐藥。不同藥物濃度作用下，耐藥株組CDR1和CDR2的錶達量相對要高于敏感株組（P＜0.05）。結論白色唸珠毬菌對氟康唑耐藥率較高，耐藥株組CDR1和CDR2錶達量高于敏感株組。
목적：조사백색념주균림상주대불강서적내약정황，연구백색념주균림상내약기인적표체여불강서내약지간적관계。방법채용M27-A2미량육탕희석법，대모원미생물실험실2010년분리보존적221주래자취진환자적담、중단뇨、분편화인식자적백색념주균진행불강서MIC치측정。이백색념주균18S RNA위내삼조，채용RT-PCR기술관찰비교내약주화민감주조재불동불강서농도하CDR1화CDR2기인적전록수평。결과221주백색념주균중，유57.47％（127주）대불강서민감，10.86％（24주）제량의뢰민감，31.67％（70주）내약。불동약물농도작용하，내약주조CDR1화CDR2적표체량상대요고우민감주조（P＜0.05）。결론백색념주구균대불강서내약솔교고，내약주조CDR1화CDR2표체량고우민감주조。
Objective ToinvestigatethefluconazoleresistanceofclinicalstrainsofCandidaalbicansandtoexplorethe relationshipbetweenclinicaldrugresistance(CDR)geneofCandidaalbicansandfluconazoleresistance.Methods Using the M27-A2 micro broth dilution method,221 strains of Candida albicans from sputum separation,interrupt urine,feces and throat swabs which conserved in our laboratory during the year 2010 were measured with fluconazole MIC value. Candida albicans 18S RNA was used as internal reference,the transcription levels of CDR1 and CDR2 gene in resistant and sensitive strain groups were compared under different fluconazole concentrations with RT-PCR technique.Results Amongtherandomlyselected221strainsofCandidaalbicans,127(57.47%)strainsweresusceptibletofluconazole,24 (10.86%)strains were sensitive dependence on dose and 70 (31.67%) strains were resistance.Under different concentration,the expression of CDR1 and CDR2 in resistant strain group was significantly higher than that of sensitive straingroup(P<0.05).Conclusion ThefluconazoleresistantrateofCandidaalbicansisrelativehighandthe expression of CDR1 and CDR2 in resistant strain group is higher than that of sensitive strain group.
