CAJ | 학술논문

目的：了解浙江省不同类型地理环境中鼠形动物蜱源立克次体的感染状况。方法2009-2011年在安吉县、金东区和天台县用夹夜法捕获鼠形动物，进行分类鉴定、无菌采集肝脾标本，并采用巢式PCR方法检测无形体属与埃立克体属16SrRNA 以及立克次体属（包括斑疹伤寒群和斑点热群）与东方体属热休克蛋白基因groEL。结果3个调查点共捕获鼠形动物14种851头，社鼠（30.32％）、黑线姬鼠（18.80％）和青毛鼠（11.75％）为主要优势种，其中金东区、安吉县和天台县的优势鼠种分别为社鼠（39.20％）、青毛鼠（32.05％）和黑线姬鼠（59.57％）。562份肝脾标本中检出立克次体48份，阳性检出率为8.54％；其中无形体属占3.38％，斑疹伤寒群占1.78％，恙虫病东方体属占1.78％，埃立克体属占1.07％，斑点热群占0.53％。金东区和安吉县无形体属检出率较高为4.76％和4.27％，斑点热群仅在天台县检出。社鼠的立克次体阳性检出率最高为14.97％。同一鼠形动物可存在多种立克次体混合感染。结论浙江省不同类型地理环境鼠形动物中广泛存在立克次体感染，不同地理位？和不同鼠形动物的蜱源立克次体检出率不同。
목적：료해절강성불동류형지리배경중서형동물비원립극차체적감염상황。방법2009-2011년재안길현、금동구화천태현용협야법포획서형동물，진행분류감정、무균채집간비표본，병채용소식PCR방법검측무형체속여애립극체속16SrRNA 이급립극차체속（포괄반진상한군화반점열군）여동방체속열휴극단백기인groEL。결과3개조사점공포획서형동물14충851두，사서（30.32％）、흑선희서（18.80％）화청모서（11.75％）위주요우세충，기중금동구、안길현화천태현적우세서충분별위사서（39.20％）、청모서（32.05％）화흑선희서（59.57％）。562빈간비표본중검출립극차체48빈，양성검출솔위8.54％；기중무형체속점3.38％，반진상한군점1.78％，양충병동방체속점1.78％，애립극체속점1.07％，반점열군점0.53％。금동구화안길현무형체속검출솔교고위4.76％화4.27％，반점열군부재천태현검출。사서적립극차체양성검출솔최고위14.97％。동일서형동물가존재다충립극차체혼합감염。결론절강성불동류형지리배경서형동물중엄범존재립극차체감염，불동지리위？화불동서형동물적비원립극차체검출솔불동。
Objective Toinvestigatethestatusoftick-borneRickettsiaeinfectionsamongmurine-likeanimalsin differentareasofZhejiangprovince.Methods Liverandspleensamplesofmurine-likeanimalscapturedthroughnight trapping method were collected from Anji,Jinhua and Tiantai County according to their geographic locations and historical detection of Rickettsiae .Nest-PCR tests were used to determine the presence of the 16S rRNA genes of Anaplasma and Ehrlichia ,and the heat shock protein genes (groEL)of Rickettsiae (including typhus and spotted fever group)and Orientiainthesesamples.Results Atotalof851murine-likeanimalsbelongingto14specieswerecaptured.The predominant species were Rattus confucianus (30.32%),Apodemus agrarius (18.80%) and Thallomys paedulcus (1 1.75%)and they were significantly different among three areas (P<0.05 ).48 Rickettsia positive were found in 562 tested samples with the positive rate of 8.54%,among which the percentage of Anaplasma,typhus group Rickettsia, Orientia,Ehrlichia and spotted fever group Rickettsia were 3.38%,1.78%,1.78%,1.07% and 0.53% respectively. The positive rates of Anaplasma in Jindong (4.76%)and Anji (4.27%)were significantly higher than that in Tiantai (P<0.05 )while the spotted fever group Rickettsia were found only in Tiantai County.Moreover,Rattus confucianus-the predominant species of Zhejiang Province-had the highest infection rate of tick-borne Rickettsiae up to 14.97%.Co-infections with several Rickettsiae were existed among the same species.Conclusion Rickettsiae infections exist widely among different areas of Zhejiang province and the positive rates are significantly different among species.