CAJ | 학술논문

目的：建立测定芪生胶囊中芍药苷含量的方法。方法采用高效液相色谱( HPLC)法测定芪生胶囊中芍药苷的含量,Kromasil C18色谱柱(4.6 mm×250 mm,5μm)；以甲醇-水(1585)为流动相；检测波长：230 nm；流速：1.0 mL·min-1。结果芍药苷在2.5~12.5μg·mL-1范围内线性关系良好,r=0.9999；平均加样回收率99.97%( n=6),RSD=0.94%。结论该方法简单,专属性强,重复性好,可有效控制芪生胶囊的质量。
목적：건립측정기생효낭중작약감함량적방법。방법채용고효액상색보( HPLC)법측정기생효낭중작약감적함량,Kromasil C18색보주(4.6 mm×250 mm,5μm)；이갑순-수(1585)위류동상；검측파장：230 nm；류속：1.0 mL·min-1。결과작약감재2.5~12.5μg·mL-1범위내선성관계량호,r=0.9999；평균가양회수솔99.97%( n=6),RSD=0.94%。결론해방법간단,전속성강,중복성호,가유효공제기생효낭적질량。
Objective To establish a content determination method for paeoniflorin in qisheng capsule. Methods The quantitative analysis of paeoniflorin in qisheng Capsule was carried out by high-performance liquid chromatography ( HPLC) . The chromatographic separation was achieved by using a Kromasil C18 chromatographic column (4. 6 mm×250 mm,5 μm) with a mobile phase consisting of methanol,water (1585) at a flow rate of 1. 0 mL·min-1 and 230 nm detection wavelength. Results The linear range was 2. 5-12. 5 μg·mL-1( r =0. 999 9). The average recovery and RSD of the method were 99. 97%and 0. 94%. Conclusion The method is accurate,specific,reproducible,which can effectively be used in quality control of paeoniflorin in qisheng capsule.