医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2014年
7期
935-937
,共3页
海力茜·陶尔大洪%古娜娜·对山别克
海力茜·陶爾大洪%古娜娜·對山彆剋
해력천·도이대홍%고나나·대산별극
恰麻古%总黄酮%分光光度法,紫外可见
恰痳古%總黃酮%分光光度法,紫外可見
흡마고%총황동%분광광도법,자외가견
Brassica rapa L.%Total flavonoids%Spectrophotometric,ultraviolet-visible
目的：通过单因素实验及正交实验探讨提取维药恰麻古中总黄酮的最佳工艺,并测定其不同产地的药材含量,为资源的合理开发利用提供科学依据。方法以乙醇为溶剂回流提取,采用紫外-可见分光光度法( UV)测定恰麻古中总黄酮的含量,并通过重复性、精密度和回收率实验考察实验方法。结果维药恰麻古中总黄酮的最佳工艺为乙醇浓度75%,提取时间2.5 h,提取温度80℃,料液比160。标准液及供试液均在510 nm波长处有最大吸收,总黄酮在0.008~0.048 mg· mL-1线性关系良好(r=0.9993),平均加样回收率99.59%；精密度实验、稳定性实验和重复性实验的RSD分别为0.022%,1.311%和1.601%,5个产地的恰麻古中,阿克苏恰麻古的黄酮含量最高,平均含量0.2802%。结论该方法操作简单,重复性好,灵敏度高,适用于恰麻古总黄酮的含量测定。
目的：通過單因素實驗及正交實驗探討提取維藥恰痳古中總黃酮的最佳工藝,併測定其不同產地的藥材含量,為資源的閤理開髮利用提供科學依據。方法以乙醇為溶劑迴流提取,採用紫外-可見分光光度法( UV)測定恰痳古中總黃酮的含量,併通過重複性、精密度和迴收率實驗攷察實驗方法。結果維藥恰痳古中總黃酮的最佳工藝為乙醇濃度75%,提取時間2.5 h,提取溫度80℃,料液比160。標準液及供試液均在510 nm波長處有最大吸收,總黃酮在0.008~0.048 mg· mL-1線性關繫良好(r=0.9993),平均加樣迴收率99.59%；精密度實驗、穩定性實驗和重複性實驗的RSD分彆為0.022%,1.311%和1.601%,5箇產地的恰痳古中,阿剋囌恰痳古的黃酮含量最高,平均含量0.2802%。結論該方法操作簡單,重複性好,靈敏度高,適用于恰痳古總黃酮的含量測定。
목적：통과단인소실험급정교실험탐토제취유약흡마고중총황동적최가공예,병측정기불동산지적약재함량,위자원적합리개발이용제공과학의거。방법이을순위용제회류제취,채용자외-가견분광광도법( UV)측정흡마고중총황동적함량,병통과중복성、정밀도화회수솔실험고찰실험방법。결과유약흡마고중총황동적최가공예위을순농도75%,제취시간2.5 h,제취온도80℃,료액비160。표준액급공시액균재510 nm파장처유최대흡수,총황동재0.008~0.048 mg· mL-1선성관계량호(r=0.9993),평균가양회수솔99.59%；정밀도실험、은정성실험화중복성실험적RSD분별위0.022%,1.311%화1.601%,5개산지적흡마고중,아극소흡마고적황동함량최고,평균함량0.2802%。결론해방법조작간단,중복성호,령민도고,괄용우흡마고총황동적함량측정。
Objective To establish the optimum parameters for the flavonoids extraction from Brassica rapa L. by single-factor and orthogonal experiments,determine the content of flavonoids in Brassica rapa L. from different origins,and provide a scientific basis for the rational development and utilization of Brassica rapa L. Methods The total flavonoids in Brassica rapa L. was extracted by ethanol reflux extraction and the content was determined by ultraviolet-visible spectrophotometry. The method was validated by tests of stability, precision and recovery. Results The optimum extracting condition for flavonoids from Brassica rapa L. was as follows: using 75% ethanol as extracting agent,setting temperature at 80 ℃,using solid-liquid ratio at 1 g60 mL and extracting for 2. 5 h. The detection wavelength was 510 nm. The linear range and the detectable limit of the method were 0. 008-0. 048 mg· mL-1(r=0. 999 3). The average extraction recovery was 99. 59%. The RSD of the precision, stability and recovery tests were 0. 022%,1. 311%,and 1. 601%,respectively. The Brassica rapa L. from Akzo contained the highest flavonoids with an average value of 0. 280 2%. Conclusion This method is simple,reproducible,sensitive and can be used to determine the total flavonoids in Brassica rapa L . .
