医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2014年
7期
931-934
,共4页
李文静%蔡德富%于庆莲%牛英才%郭丽娜%洪博
李文靜%蔡德富%于慶蓮%牛英纔%郭麗娜%洪博
리문정%채덕부%우경련%우영재%곽려나%홍박
红花%羟基红花黄色素A%提取工艺%正交实验%肝纤维化
紅花%羥基紅花黃色素A%提取工藝%正交實驗%肝纖維化
홍화%간기홍화황색소A%제취공예%정교실험%간섬유화
Carthamus tinctorius L.%Hydroxysafflor yellow A%Extraction technology%Orthogonal experiment%Hepatic fibrosis
目的:采用正交实验法优选有抗肝纤维化作用的红花水溶性成分的最佳提取工艺。方法采用高效液相色谱法和酶联免疫吸附法,以羟基红花黄色素A含量、浸膏得率及抗肝纤维化作用为综合评判指标,通过L9(3)4正交实验设计,考察提取溶剂、溶剂用量、回流时间和回流次数等因素对提取工艺的影响。结果具有抗肝纤维化作用的红花水溶性成分的最佳提取工艺为用10倍量10%乙醇,提取2次,每次0.5 h。结论该工艺科学可行,对红花抗肝纤维化水溶性成分的提取生产工艺具有一定的指导和参考意义。
目的:採用正交實驗法優選有抗肝纖維化作用的紅花水溶性成分的最佳提取工藝。方法採用高效液相色譜法和酶聯免疫吸附法,以羥基紅花黃色素A含量、浸膏得率及抗肝纖維化作用為綜閤評判指標,通過L9(3)4正交實驗設計,攷察提取溶劑、溶劑用量、迴流時間和迴流次數等因素對提取工藝的影響。結果具有抗肝纖維化作用的紅花水溶性成分的最佳提取工藝為用10倍量10%乙醇,提取2次,每次0.5 h。結論該工藝科學可行,對紅花抗肝纖維化水溶性成分的提取生產工藝具有一定的指導和參攷意義。
목적:채용정교실험법우선유항간섬유화작용적홍화수용성성분적최가제취공예。방법채용고효액상색보법화매련면역흡부법,이간기홍화황색소A함량、침고득솔급항간섬유화작용위종합평판지표,통과L9(3)4정교실험설계,고찰제취용제、용제용량、회류시간화회류차수등인소대제취공예적영향。결과구유항간섬유화작용적홍화수용성성분적최가제취공예위용10배량10%을순,제취2차,매차0.5 h。결론해공예과학가행,대홍화항간섬유화수용성성분적제취생산공예구유일정적지도화삼고의의。
Objective To optimize the extraction process of anti-hepatic fibrosis water-soluble component from Carthamus tinctorius L. via the orthogonal method. Methods The influences of type and content of solvent,extraction time,and extraction frequency on processing were investigated by assessing the content of hydroxysafflor yellow A,yield of extraction and anti-hepatic fibrosis potency via the high performance liquid chromatography and enzyme-linked immunosorbent assay by L9(3)4 orthogonal test. Results The optimum extracting condition for the anti-hepatic fibrosis water-soluble component from carthamus tinctorius L. was as follows: extracting with 10 times the volume of 10% ethanol for twice, 0. 5 h in each. Conclusion The process is scientific and feasible, which serves as a guideline for the production of the anti-hepatic fibrosis water-soluble components.