CAJ | 학술논문

目的：研究湖北海棠叶水煎剂对I型单纯疱疹病毒(HSV-1)所致结膜炎的治疗作用。方法采用湖北海棠叶水煎剂作为实验样品,用利巴韦林滴眼剂作对照。分别以HSV-1感染Vero细胞和家兔眼结膜,通过观察细胞病变效应判断药物抑制病毒复制的环节。对家兔眼结膜炎疾病模型进行眼结膜组织病理学检查,观察低、中、高浓度湖北海棠叶水煎剂对HSV-1兔眼结膜炎的治疗作用。结果体外实验中,湖北海棠叶水煎剂对HSV-1增殖无抑制作用。整体动物实验中,眼部给予湖北海棠叶水煎剂3,6 kg·L-1,连续给药7 d,家兔眼结膜病理性炎症反应明显减弱甚至消失,与0.9%氯化钠溶液对照组比较,差异有统计学意义(P<0.05)。中、高剂量组家兔眼结膜炎治疗有效率分别为83.3%和100.0%。结论湖北海棠叶水煎剂可减轻HSV-1所致家兔结膜炎炎性反应。
목적：연구호북해당협수전제대I형단순포진병독(HSV-1)소치결막염적치료작용。방법채용호북해당협수전제작위실험양품,용리파위림적안제작대조。분별이HSV-1감염Vero세포화가토안결막,통과관찰세포병변효응판단약물억제병독복제적배절。대가토안결막염질병모형진행안결막조직병이학검사,관찰저、중、고농도호북해당협수전제대HSV-1토안결막염적치료작용。결과체외실험중,호북해당협수전제대HSV-1증식무억제작용。정체동물실험중,안부급여호북해당협수전제3,6 kg·L-1,련속급약7 d,가토안결막병이성염증반응명현감약심지소실,여0.9%록화납용액대조조비교,차이유통계학의의(P<0.05)。중、고제량조가토안결막염치료유효솔분별위83.3%화100.0%。결론호북해당협수전제가감경HSV-1소치가토결막염염성반응。
Objective To investigate the therapeutic effect of the Hubei Wingnut ( Malus hapehensis ) leaf decoction (MHD) against conjunctivitis infected with human simplex virus type I (HSV-1). Methods Malus hupehcnsis decoction was used as the active treatment and ribavirin ( RBV) eye drop was used as the positive control. Both of the Vero cells and rabbit eye conjunctiva were infected with HSV-1. The effect and mechanism of the MHD on viral replication was determined by observing the cytopathic effect ( CPE) . The efficacy of MHD at different doses on the rabbit viral conjunctivitis was examined by pathological changes of eye conjunctiva tissues. Results MHD did not inhibit the proliferation of HSV-1 in vitro. The inflammatory reactions of rabbit viral conjunctivitis caused by HSV-1 were obviously attenuated or disappeared after treatment with MHD at 6 kg·L-1 and 3 kg·L-1 for 7 consecutive days,compared with the negative control of 0. 9% NaCl. The curative rate of MHD at the middle and high doses was 83. 3% and 100. 0%, respectively. Conclusion MHD has the potential for treating eye conjunctivitis caused by HSV-1 by relieving inflammation.