CAJ | 학술논문

目的：观察芍药苷（PF）对血管性痴呆大鼠学习记忆能力及乙酰胆碱酯酶（AchE）、超氧化物歧化酶（SOD）水平的影响。方法50只Wistar大鼠随机分为假手术组、模型组、给药组（芍药苷低剂量组12．5 mg/kg、中剂量组25 mg/kg、高剂量组50 mg/kg），每组10只。模型组与给药组采用反复夹闭双侧颈总动脉（CCA）结合腹腔注射硝普钠法造模，假手术组不阻断CCA及注射硝普钠。造模后第2天给药组予灌胃给药，模型组与假手术组给予生理盐水灌胃，每天1次，连续30 d。应用Morris水迷宫实验、跳台实验测定大鼠学习记忆能力，并比较各组AchE、SOD活性。结果（1）Morris水迷宫实验：模型组和假手术组定位航行实验中逃避潜伏期均逐渐缩短，第5天明显比第1天短（ P ＜0．05）；与假手术组比较，模型组潜伏期在各个时间点均延长，而穿过平台的次数明显减少（ P ＜0．01）；与模型组比较，给药组潜伏期呈缩短趋势，第5天比第1天短（ P ＜0．05）；穿过平台的次数明显增加（ P ＜0．05或P ＜0．01）。（2）跳台实验：与假手术组比较，模型组错误次数明显增加，而潜伏期明显缩短（ P ＜0．01）；与模型组比较，3个给药组的错误次数均明显降低，潜伏期均高于模型组（ P ＜0．05或P ＜0．01）。与低、中剂量组比较，高剂量组潜伏期延长更显著（ P ＜0．01）。（3）脑组织匀浆中AchE、SOD活性：与假手术组比较，模型组AchE活性增高、SOD活性降低（ P均＜0．01）；与模型组比较，低、中、高剂量组AchE活性明显降低、SOD活性均升高（ P ＜0．05或P ＜0．01）；与低剂量组相比，高剂量组AchE活性降低（ P ＜0．05），SOD活性明显升高（ P ＜0．01）；与中剂量组相比，高剂量组SOD活性升高（ P ＜0．05）。结论芍药苷对血管性痴呆大鼠学习记忆能力障碍有改善作用，其机制之一可能是基于抑制AchE活性，增加SOD的活性。目的：觀察芍藥苷（PF）對血管性癡呆大鼠學習記憶能力及乙酰膽堿酯酶（AchE）、超氧化物歧化酶（SOD）水平的影響。方法50隻Wistar大鼠隨機分為假手術組、模型組、給藥組（芍藥苷低劑量組12．5 mg/kg、中劑量組25 mg/kg、高劑量組50 mg/kg），每組10隻。模型組與給藥組採用反複夾閉雙側頸總動脈（CCA）結閤腹腔註射硝普鈉法造模，假手術組不阻斷CCA及註射硝普鈉。造模後第2天給藥組予灌胃給藥，模型組與假手術組給予生理鹽水灌胃，每天1次，連續30 d。應用Morris水迷宮實驗、跳檯實驗測定大鼠學習記憶能力，併比較各組AchE、SOD活性。結果（1）Morris水迷宮實驗：模型組和假手術組定位航行實驗中逃避潛伏期均逐漸縮短，第5天明顯比第1天短（ P ＜0．05）；與假手術組比較，模型組潛伏期在各箇時間點均延長，而穿過平檯的次數明顯減少（ P ＜0．01）；與模型組比較，給藥組潛伏期呈縮短趨勢，第5天比第1天短（ P ＜0．05）；穿過平檯的次數明顯增加（ P ＜0．05或P ＜0．01）。（2）跳檯實驗：與假手術組比較，模型組錯誤次數明顯增加，而潛伏期明顯縮短（ P ＜0．01）；與模型組比較，3箇給藥組的錯誤次數均明顯降低，潛伏期均高于模型組（ P ＜0．05或P ＜0．01）。與低、中劑量組比較，高劑量組潛伏期延長更顯著（ P ＜0．01）。（3）腦組織勻漿中AchE、SOD活性：與假手術組比較，模型組AchE活性增高、SOD活性降低（ P均＜0．01）；與模型組比較，低、中、高劑量組AchE活性明顯降低、SOD活性均升高（ P ＜0．05或P ＜0．01）；與低劑量組相比，高劑量組AchE活性降低（ P ＜0．05），SOD活性明顯升高（ P ＜0．01）；與中劑量組相比，高劑量組SOD活性升高（ P ＜0．05）。結論芍藥苷對血管性癡呆大鼠學習記憶能力障礙有改善作用，其機製之一可能是基于抑製AchE活性，增加SOD的活性。
목적：관찰작약감（PF）대혈관성치태대서학습기억능력급을선담감지매（AchE）、초양화물기화매（SOD）수평적영향。방법50지Wistar대서수궤분위가수술조、모형조、급약조（작약감저제량조12．5 mg/kg、중제량조25 mg/kg、고제량조50 mg/kg），매조10지。모형조여급약조채용반복협폐쌍측경총동맥（CCA）결합복강주사초보납법조모，가수술조불조단CCA급주사초보납。조모후제2천급약조여관위급약，모형조여가수술조급여생리염수관위，매천1차，련속30 d。응용Morris수미궁실험、도태실험측정대서학습기억능력，병비교각조AchE、SOD활성。결과（1）Morris수미궁실험：모형조화가수술조정위항행실험중도피잠복기균축점축단，제5천명현비제1천단（ P ＜0．05）；여가수술조비교，모형조잠복기재각개시간점균연장，이천과평태적차수명현감소（ P ＜0．01）；여모형조비교，급약조잠복기정축단추세，제5천비제1천단（ P ＜0．05）；천과평태적차수명현증가（ P ＜0．05혹P ＜0．01）。（2）도태실험：여가수술조비교，모형조착오차수명현증가，이잠복기명현축단（ P ＜0．01）；여모형조비교，3개급약조적착오차수균명현강저，잠복기균고우모형조（ P ＜0．05혹P ＜0．01）。여저、중제량조비교，고제량조잠복기연장경현저（ P ＜0．01）。（3）뇌조직균장중AchE、SOD활성：여가수술조비교，모형조AchE활성증고、SOD활성강저（ P균＜0．01）；여모형조비교，저、중、고제량조AchE활성명현강저、SOD활성균승고（ P ＜0．05혹P ＜0．01）；여저제량조상비，고제량조AchE활성강저（ P ＜0．05），SOD활성명현승고（ P ＜0．01）；여중제량조상비，고제량조SOD활성승고（ P ＜0．05）。결론작약감대혈관성치태대서학습기억능력장애유개선작용，기궤제지일가능시기우억제AchE활성，증가SOD적활성。
Objective To observe the effect of paeoniflorin ( PF) on the vascular dementia rats learning and memory ability, and acetylcholinesterase (AchE), superoxide dismutase (SOD) level.Methods Fifty Wistar rats were randomly di-vided into sham operation group, model group, drug group (paeoniflorin low dose group with 12.5 mg/kg, middle dose group with 25 mg/kg, high dose group with 50 mg/kg), with 10 rats in each group.The model group and the treatment group by re-peated clamping of bilateral common carotid artery ( CCA) model combined with intraperitoneal injection of sodium nitroprus-side, sham operation group did not block the CCA and injection of sodium nitroprusside .After second day of establish the mode , treatment group was given intragastric administration , the model group and the sham operation group were given normal saline, 1 times a day, continuous 30 d.Application of Morris water maze test , step-down test the learning and memory ability of rats, and comparison of AchE, SOD activity.Results (1) Morris water maze experiment:model group and sham operation group positioning navigation test in escape latency gradually shortened , fifth days shorter than first days ( P <0.05); com-pared with the sham operation group , model group were prolonged incubation at each time point , and through the platform was significantly reduced ( P <0.01); compared with the model group , drug group latency showed a trend of shortening , fifth days more shorter than first days ( P <0.05); times across the platform significantly increased ( P <0.05 or P <0.01). (2) In step-down test:compared with the sham operation group , model group, the number of errors increased significantly , and was significantly shortened ( P <0.01);compared with the model group , the 3 groups were significantly decreased the number of errors, the latency was higher than that in the model group ( P <0.05 or P <0.01).Compared with the low,mid-dle dose group, high dose group had significantly more prolonged latency ( P <0.01).(3) AchE, SOD activity of brain ho-mogenates:compared with the sham operation group , model group, AchE decreased, SOD activity increased ( P <0.01);compared with the model group , low, middle and high dose group's AchE activity decreased , SOD activity increased ( P <0.05 or P <0.01);compared with the low dose group , high dose group AchE activity decreased ( P <0.05), the activity of SOD increased significantly ( P <0.01); compared with middle dose group, high dose group's activity of SOD increased (P <0.05).Conclusion Paeoniflorin improved vascular dementia rats learning and memory ability , one of the possible mechanisms is on the basis of inhibition of AchE activity to increase the activity of SOD .