CAJ | 학술논문

目的：探讨exendin-4（Ex-4）预处理对大鼠缺血/再灌注（I/R）损伤的影响。方法雄性SD大鼠55只随机分为4组：假手术组（SO）（ n ＝10），缺血/再灌注（I/R）组（ n ＝15），Ex-4＋I/R （Ex-4-I/R）组（ n ＝15）， Ex-4＋exendin （9-39）[Ex-4-Ex（9-39）-I/R]组（ n ＝15）。除SO组外，其余3组大鼠均行冠状动脉左前降支结扎，缺血30 min后再灌4 h，Ex-4-I/R组在I/R前给予Ex-4预处理，Ex-4-Ex（9-39）-I/R组在I/R前给予Ex-4和Ex（9-39）预处理。再灌注4h后进行心肌梗死面积评估，并取颈动脉血进行心肌酶、心肌氧化指标、心肌高迁移率族蛋白1（HMGB1）水平检测。结果缺血再灌注4h后，与I/R组比较，Ex-4-I/R组心肌梗死面积减少，分别为（54．1±3．0）％和（25．8±1．0）％，差异有统计学意义（ P ＜0．05）；与Ex-4-I/R组比较，Ex-4-Ex（9-39）-I/R组心肌梗死面积增大，为（47．8±3．0）％，差异有统计学意义（ P ＜0．05）。与SO组比较，I/R组LDH、CK、MDA水平明显增加，HMGB1表达明显升高（ P ＜0．05），而SOD水平明显下降（ P ＜0．05）；与I/R组比较，Ex-4-I/R组各项指标明显改善，差异有统计学意义（P ＜0．05）；与Ex-4-I/R组比较，Ex-4-Ex（9-39）-I/R组中各项指标的改善均被抑制，差异有统计学意义（ P ＜0．05）。结论 Ex-4预处理可能通过抑制HMGB1的表达进而减轻大鼠心肌I/R损伤。
목적：탐토exendin-4（Ex-4）예처리대대서결혈/재관주（I/R）손상적영향。방법웅성SD대서55지수궤분위4조：가수술조（SO）（ n ＝10），결혈/재관주（I/R）조（ n ＝15），Ex-4＋I/R （Ex-4-I/R）조（ n ＝15）， Ex-4＋exendin （9-39）[Ex-4-Ex（9-39）-I/R]조（ n ＝15）。제SO조외，기여3조대서균행관상동맥좌전강지결찰，결혈30 min후재관4 h，Ex-4-I/R조재I/R전급여Ex-4예처리，Ex-4-Ex（9-39）-I/R조재I/R전급여Ex-4화Ex（9-39）예처리。재관주4h후진행심기경사면적평고，병취경동맥혈진행심기매、심기양화지표、심기고천이솔족단백1（HMGB1）수평검측。결과결혈재관주4h후，여I/R조비교，Ex-4-I/R조심기경사면적감소，분별위（54．1±3．0）％화（25．8±1．0）％，차이유통계학의의（ P ＜0．05）；여Ex-4-I/R조비교，Ex-4-Ex（9-39）-I/R조심기경사면적증대，위（47．8±3．0）％，차이유통계학의의（ P ＜0．05）。여SO조비교，I/R조LDH、CK、MDA수평명현증가，HMGB1표체명현승고（ P ＜0．05），이SOD수평명현하강（ P ＜0．05）；여I/R조비교，Ex-4-I/R조각항지표명현개선，차이유통계학의의（P ＜0．05）；여Ex-4-I/R조비교，Ex-4-Ex（9-39）-I/R조중각항지표적개선균피억제，차이유통계학의의（ P ＜0．05）。결론 Ex-4예처리가능통과억제HMGB1적표체진이감경대서심기I/R손상。
Objective To investigate the effect of exendin 4 ( Ex-4) preconditioning on rat ischemia/reperfusion ( I/R) damage.Methods Fifty-five male SD rats were randomly divided into 4 groups:sham operation group (SO) ( n =10), ischemia /reperfusion (I/R) group ( n =15),EX4+I/R (Ex-4-I/R) group (n=15), Ex-4+exendin (9-39)[Ex-4-Ex (9-39)-I/R] group ( n =15).Except SO group, the other 3 group rats underwent left anterior descending coronary artery ligation, after 30 min ischemia, reperfusion of 4 h, Ex-4-I/R group was given Ex-4 before I/R pretreatment, Ex-4-Ex(9-39)-I/R group was given Ex-4 and Ex-(9-39) preconditioning before I/R group.After reperfusion for 4h, then assessed of the my-ocardial infarction area , and get the carotid artery blood sample to test the myocardial enzyme , myocardial oxidative index , myocardial high mobility group protein 1 (HMGB1) levels.Results 4h after reperfusion, compared with group I/R, Ex-4-I/R group reduced myocardial infarct size more obviously , (54.1 ±3)% and (25.8 ±1)% respectively, the difference was statistically significant ( P <0.05) compared with Ex-4-I/R group, Ex-4-Ex(9-39)-I/R group myocardial infarction area in-creases, as of (47.8 ±3.0)%,the difference was statistically significant ( P <0.05).Compared with SO group, I/R group's LDH, CK, MDA levels were significantly increased, HMGB1 expression was significantly increased ( P <0.05), and SOD level decreased significantly ( P <0.05);compared with I/R group, Ex-4-I/R group's indicators improved significantly , the difference was statistically significant ( P <0.05);compared with the Ex-4-I/R group, Ex-4-Ex(9-39)-I/R group's indica-tors improvement were suppressed , the difference was statistically significant ( P <0.05).Conclusion It demonstrated that the Ex-4 preconditioning may inhibit the expression of HMGB 1 and reduce the myocardial I/R injury in rats .