中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2009年
9期
30-31
,共2页
p16INK4a蛋白%免疫组化%喉鳞状细胞癌
p16INK4a蛋白%免疫組化%喉鱗狀細胞癌
p16INK4a단백%면역조화%후린상세포암
P16INK4A Protein%Immunohistochemistry%Throat squamous carcinoma
目的 探讨喉鳞状细胞癌、喉鳞状上皮不典型增生及喉慢性炎中p16INK4a蛋白表达水平的变化及意义.方法 应用免疫组化方法检测86例喉鳞状上皮病变(喉鳞状细胞癌16例、喉上皮不典型增生48例,喉慢性炎22例)组织中p16INK4a蛋白的表达,进行半定量分析及统计学检验.结果 16例喉鳞状细胞癌中,p16INK4a全部阳性表达;48例喉上皮内瘤变中,35例p16INK4a阳性表达,阳性率为72.92%,其中轻度不典型增生,中度不典型增生,重度不典型增生p16INK4a阳性表达率分别是33.3%(4/12)、75%(15/20)、100%(16/16),且三者间差异有统计学意义(P＜0.05).22例喉慢性炎中,p16INK4a阳性表达6例,阳性表达率为27.27%.p16INK4a阳性蛋白在喉鳞状细胞癌、喉鳞状上皮不典型增生及喉慢性炎组织中表达水平依次降低,且差异有统计学意义(P＜0.05).结论 喉鳞状细胞癌、喉鳞状上皮不典型增生中p16INK4a阳性表达明显高于喉慢性炎.p16INK4a在喉鳞状细胞癌、喉鳞状上皮不典型增生中过表达,提示其在喉鳞状细胞癌、喉鳞状上皮不典型增生发生发展中起重要作用.
目的 探討喉鱗狀細胞癌、喉鱗狀上皮不典型增生及喉慢性炎中p16INK4a蛋白錶達水平的變化及意義.方法 應用免疫組化方法檢測86例喉鱗狀上皮病變(喉鱗狀細胞癌16例、喉上皮不典型增生48例,喉慢性炎22例)組織中p16INK4a蛋白的錶達,進行半定量分析及統計學檢驗.結果 16例喉鱗狀細胞癌中,p16INK4a全部暘性錶達;48例喉上皮內瘤變中,35例p16INK4a暘性錶達,暘性率為72.92%,其中輕度不典型增生,中度不典型增生,重度不典型增生p16INK4a暘性錶達率分彆是33.3%(4/12)、75%(15/20)、100%(16/16),且三者間差異有統計學意義(P＜0.05).22例喉慢性炎中,p16INK4a暘性錶達6例,暘性錶達率為27.27%.p16INK4a暘性蛋白在喉鱗狀細胞癌、喉鱗狀上皮不典型增生及喉慢性炎組織中錶達水平依次降低,且差異有統計學意義(P＜0.05).結論 喉鱗狀細胞癌、喉鱗狀上皮不典型增生中p16INK4a暘性錶達明顯高于喉慢性炎.p16INK4a在喉鱗狀細胞癌、喉鱗狀上皮不典型增生中過錶達,提示其在喉鱗狀細胞癌、喉鱗狀上皮不典型增生髮生髮展中起重要作用.
목적 탐토후린상세포암、후린상상피불전형증생급후만성염중p16INK4a단백표체수평적변화급의의.방법 응용면역조화방법검측86례후린상상피병변(후린상세포암16례、후상피불전형증생48례,후만성염22례)조직중p16INK4a단백적표체,진행반정량분석급통계학검험.결과 16례후린상세포암중,p16INK4a전부양성표체;48례후상피내류변중,35례p16INK4a양성표체,양성솔위72.92%,기중경도불전형증생,중도불전형증생,중도불전형증생p16INK4a양성표체솔분별시33.3%(4/12)、75%(15/20)、100%(16/16),차삼자간차이유통계학의의(P＜0.05).22례후만성염중,p16INK4a양성표체6례,양성표체솔위27.27%.p16INK4a양성단백재후린상세포암、후린상상피불전형증생급후만성염조직중표체수평의차강저,차차이유통계학의의(P＜0.05).결론 후린상세포암、후린상상피불전형증생중p16INK4a양성표체명현고우후만성염.p16INK4a재후린상세포암、후린상상피불전형증생중과표체,제시기재후린상세포암、후린상상피불전형증생발생발전중기중요작용.
Objective To study the expression of P16INK4A protein in throat squamous carcinoma,throat squamous cell atypia-hyperplasia and throat chronic laryngitis,and its significance.Methods The expression of P16INK4A protein was detected in 86 throat samples(throat squamous carcinoma 16 cases,atypia hyperplasia 48 cases,throat chronic laryngitis 22 cases)by immunohistochemical staining.Results P16INK4A protein was positive in all 16 cases of throat squamous carcinoma.The positive expression of P16INK4A was de tected in 35 of 48 cases of throat squamous cell atypia-hyperplasia,4/12 in low-grade atypia-hyperplasia,15/20 in middle-grade atypia-hyperplasia,16/16 in high-grade atypia-hyperplasia.The positive expression of p16 was detected in 6 of 22 cases of chronic laryngitis.P16INK4A increased with an increasing order in laryngitis,atypiahyperplasia to squamous carcinoma,with significant difference among the 3 groups (P＜0.05).Conclusion P161NK4A over expresses in squamous carcinoma and precancerous lesions.Which plays an important role in carcinogenesis of squamous carcinoma.
