CAJ | 학술논문

建立了亲水/反相二维色谱用于制备桔梗中三萜皂苷单体的方法。桔梗经水煮醇沉、反相和亲水两种模式的固相萃取后得到三萜皂苷类组分。选定 XAmide色谱柱（150 mm×20 mm，5μm），以乙腈和水为流动相，在亲水色谱模式下进行组分制备。选择时间触发模式，以1 min为单位进行馏分收集，得到6~25 min 之间的20个三萜皂苷精细组分。以第18个馏分（JG23）为例，在反相色谱模式下采用 Atlantis Prep T3色谱柱（100 mm×30 mm，5μm）制备，得到两个单体化合物。通过质谱和核磁共振对其进行定性，确定分别为deapi-platycoside E和platyco-side E。实验结果表明，该制备方法具有好的正交选择性，对于复杂样品中三萜皂苷类化合物的分离纯化有一定的借鉴意义。
건립료친수/반상이유색보용우제비길경중삼첩조감단체적방법。길경경수자순침、반상화친수량충모식적고상췌취후득도삼첩조감류조분。선정 XAmide색보주（150 mm×20 mm，5μm），이을정화수위류동상，재친수색보모식하진행조분제비。선택시간촉발모식，이1 min위단위진행류분수집，득도6~25 min 지간적20개삼첩조감정세조분。이제18개류분（JG23）위례，재반상색보모식하채용 Atlantis Prep T3색보주（100 mm×30 mm，5μm）제비，득도량개단체화합물。통과질보화핵자공진대기진행정성，학정분별위deapi-platycoside E화platyco-side E。실험결과표명，해제비방법구유호적정교선택성，대우복잡양품중삼첩조감류화합물적분리순화유일정적차감의의。
A two-dimensional(2-D)preparative liquid chromatography method was developed for the preparation of triterpene saponins from Platycodon grandiflorum using hydrophilic interaction liquid chromatography( HILIC)coupled with reversed phase liquid chromatography ( RPLC). At first,the crude extract was obtained from Platycodon grandiflorum by boiled alco-hol precipitation. Then,the concentrated crude extracts were continuously pretreated using sol-id phase extraction( SPE)under reversed-phase and hydrophilic-phase modes to remove the impurities. Subsequently,XAmide column(150 mm×20 mm,5 μm)was selected to separate the triterpene saponin constituents under HILIC mode using water and acetonitrile as mobile phases. From 6 min to 25 min,each fraction was collected per minute under time-triggered mode and 20 fractions were collected. The 18th fraction( JG23)was selected for further purifi-cation. The column of Atlantis PrepT3(100 mm×30 mm,5 μm)was chosen and two monomer-ic compounds were obtained. The two compounds with over 90% purity were identified as dea-pi-platycoside E and platycoside E with mass spectrometry( MS)and nuclear magnetic reso-nance( NMR). This 2-D HILIC-RPLC method with high orthogonality can be used in the prepa-ration of triterpene saponins from natural products.