CAJ | 학술논문

研究了不同温度（4、8、12、16、20、24℃和温度突变）下干露对刺参体壁非特异性免疫的影响。结果表明，在温度20和24℃条件下干露24 h后，刺参全部死亡，其余处理组刺参成活率为100％。刺参体壁水解酶类和抗氧化指标对于不同温度下干露胁迫具有不同的响应：（1）在本研究的温度范围内，刺参体壁酸性磷酸酶（ACP）、碱性磷酸酶（AKP）活性总体随温度升高而增高，随干露时间的延长而逐渐下降；（2）刺参体壁中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化（T -AOC）活性等抗氧化指标呈现随温度的升高先增强，再逐渐下降的趋势，以16℃为最高。对于干露胁迫时间的响应，抗氧化酶活性等则在低温时（4～16℃）呈现先升高，然后逐渐下降的趋势，在高温（20和24℃）干露胁迫下，则呈现持续下降的趋势。（3）而模拟干法运输的刺参非特异性免疫指标的响应，总体上与12～16℃下干露胁迫相当，而且4～24 h之内，其A K P、SOD活性和T -AOC和MDA数值基本保持稳定。本研究结果表明，刺参干法运输中保持适当的低温可有效降低刺参的胁迫反应，提高刺参运输的成活率。
연구료불동온도（4、8、12、16、20、24℃화온도돌변）하간로대자삼체벽비특이성면역적영향。결과표명，재온도20화24℃조건하간로24 h후，자삼전부사망，기여처리조자삼성활솔위100％。자삼체벽수해매류화항양화지표대우불동온도하간로협박구유불동적향응：（1）재본연구적온도범위내，자삼체벽산성린산매（ACP）、감성린산매（AKP）활성총체수온도승고이증고，수간로시간적연장이축점하강；（2）자삼체벽중초양화물기화매（SOD）、과양화경매（CAT）화총항양화（T -AOC）활성등항양화지표정현수온도적승고선증강，재축점하강적추세，이16℃위최고。대우간로협박시간적향응，항양화매활성등칙재저온시（4～16℃）정현선승고，연후축점하강적추세，재고온（20화24℃）간로협박하，칙정현지속하강적추세。（3）이모의간법운수적자삼비특이성면역지표적향응，총체상여12～16℃하간로협박상당，이차4～24 h지내，기A K P、SOD활성화T -AOC화MDA수치기본보지은정。본연구결과표명，자삼간법운수중보지괄당적저온가유효강저자삼적협박반응，제고자삼운수적성활솔。
Sea cucumber A postichopus j aponicus with body weight of (50 ± 5)g were exposed to differ-ent desiccation temperatures (4 ℃ ,8 ℃ ,12 ℃ ,16 ℃ ,20 ℃ ,24 ℃ and sudden temperature change) for 0h ,2 h ,4 h ,8 h ,12 h ,24 h and sampled for body wall to study changes of weight loss ratio ,coelomic fluid pH and osmotic pressure and six non - specific immune indices including acid phosphatase (ACP) ,alkaline phosphatase(AKP) ,superoxide dismutase(SOD) ,catalase(CAT) ,the total anti-oxi-dation capability(T -AOC) and MDA contents .The results indicated that all the sea cucumber died at 20 ℃ and 24 ℃ after desiccation for 24 h .The survival rates of the other treatments were 100 .0% . T he hydrolases and antioxidases responded differently to temperature and desiccation .T he activity of ACP and AKP changed positively with temperature change ,but negatively with exposure time .The ac-tivities of SOD ,CAT and T -AOC increased firstly and then decreased gradually with temperature increase ,with the maximum at 16 ℃ .And they increased firstly and decreased gradu-ally at lower temperatures(4~16 ℃) ,but dropped continually at higher temperatures(20~24 ℃)with the time .Under sudden desiccation temperature changes ,the non -specific immune indices of sea cu-cumber changed similarly with that at 12 and 16 ℃ generally .Within 4~24 h ,activities of AKP and SOD ,T -AOC and MDA contents almost kept stable .It can be concluded that it’s beneficial for im-munity of sea cucumber to keep appropriate low temperature during no -water transportation .