CAJ | 학술논문

目的：探讨金龙胶囊对肺腺癌A549移植瘤生长的抑制作用。方法建立裸鼠移植瘤模型，随机分成阴性对照组（ A组）、单药金龙胶囊组（B组）、单药顺铂组（C组）和联合用药组（D组）4组，连续用药4周。观察肿瘤生长情况，计算抑瘤率；免疫组化法检测移植瘤微血管密度（microvessel density，MVD）；RT-PCR分析各组治疗后VEGF及凋亡基因Bcl-2mRNA表达情况。结果与阴性对照组比较，金龙胶囊治疗组（ B、D组）抑瘤率分别为26.94％、51.61％，联合用药组（ D组）效果最佳。治疗组微血管密度分别为18.83±0.75、14.17±0.75，联合用药组最为显著，而单药顺铂组与对照组在抑瘤率和微血管密度表达上均无差异（ P＞0.05）。金龙胶囊治疗组（B、D组）Bcl-2mRNA表达较对照组有显著差异（P＜0.001），但两组之间无差异（P＝0.369）；四组之间在VEGFmRNA表达上无明显差异（ P＞0.05）。结论金龙胶囊对荷A549裸鼠移植瘤有抑制作用，联合顺铂用药有显著协同作用，其作用机制可能与抑制肿瘤微血管形成、诱导细胞凋亡增加有关。
목적：탐토금룡효낭대폐선암A549이식류생장적억제작용。방법건립라서이식류모형，수궤분성음성대조조（ A조）、단약금룡효낭조（B조）、단약순박조（C조）화연합용약조（D조）4조，련속용약4주。관찰종류생장정황，계산억류솔；면역조화법검측이식류미혈관밀도（microvessel density，MVD）；RT-PCR분석각조치료후VEGF급조망기인Bcl-2mRNA표체정황。결과여음성대조조비교，금룡효낭치료조（ B、D조）억류솔분별위26.94％、51.61％，연합용약조（ D조）효과최가。치료조미혈관밀도분별위18.83±0.75、14.17±0.75，연합용약조최위현저，이단약순박조여대조조재억류솔화미혈관밀도표체상균무차이（ P＞0.05）。금룡효낭치료조（B、D조）Bcl-2mRNA표체교대조조유현저차이（P＜0.001），단량조지간무차이（P＝0.369）；사조지간재VEGFmRNA표체상무명현차이（ P＞0.05）。결론금룡효낭대하A549라서이식류유억제작용，연합순박용약유현저협동작용，기작용궤제가능여억제종류미혈관형성、유도세포조망증가유관。
Objective To explore the effect of Jin Long Capsule with or without cisplatin ( DDP) on tumor growth of lung adenocarcinoma A549 cell xenografts in nude mice .Methods After a model of human lung cancer established by subcutaneous transplantation of A 549 cells in nude mice, 24 mice were randomly divided into control group (A group), Jin Long Capsule group(B group), DDP group(C group) and combination treatment group(D group).Treatment continued for 4 weeks.Inhibition rates were evaluated, tumor micro-vessel density(MVD) were determined by immune-histochemistry, the mRNA expression of VEGF and apoptosis-associated gene(Bcl-2) were assessed by RT-PCR analyses.Results The tumor growth inhibition rates of Jin Long Capsule treatment groups ( B、D groups ) were 26.94% and 51.61%respectively, and more effect in combination group .The expression of MVD in two groups above-mentioned were 18.83 ±0.75 and 14.17 ± 0.75 respectively.No statistically difference were seen between DDP group and control group .Compared to control group, RT-PCR analyses revealed a decrease in expression of Bcl-2 genes in A549 tumors by Jin Long Capsule treatment groups ( B、D groups ) , and no statistically difference in these two groups .Either group showed no statistically difference in VEGF mRNA expression .Conclusion Jin Long Capsule has inhibitory effect , and has synergetic effect with traditional chemotherapy on tumor growth of lung adencarcinoma A 549 cell xenografts in nude mice through inhibiting angiogenesis , and may enhance the sensitivity of A 549 cells to DDP by inducing cell apoptosis .