食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
9期
31-35
,共5页
枇杷核%多酚%分光光度法
枇杷覈%多酚%分光光度法
비파핵%다분%분광광도법
Loquat nuclear%polyphenol%spectrophotometry
采用乙醇-水溶液超声辅助提取法提取枇杷核中的总多酚，并选择用福林酚显色法测定其多酚的含量。利用单因素及正交试验得到显色与提取的最佳条件。得到显色的优化条件为：10%的碳酸钠1.5 mL，20%的福林酚3.5 mL，显色时间为70 min。得到提取的优化条件为：乙醇浓度为50%，提取温度为80℃，料液比为1∶20（g/mL），提取时间为120 min。该方法简便，准确，快捷。
採用乙醇-水溶液超聲輔助提取法提取枇杷覈中的總多酚，併選擇用福林酚顯色法測定其多酚的含量。利用單因素及正交試驗得到顯色與提取的最佳條件。得到顯色的優化條件為：10%的碳痠鈉1.5 mL，20%的福林酚3.5 mL，顯色時間為70 min。得到提取的優化條件為：乙醇濃度為50%，提取溫度為80℃，料液比為1∶20（g/mL），提取時間為120 min。該方法簡便，準確，快捷。
채용을순-수용액초성보조제취법제취비파핵중적총다분，병선택용복림분현색법측정기다분적함량。이용단인소급정교시험득도현색여제취적최가조건。득도현색적우화조건위：10%적탄산납1.5 mL，20%적복림분3.5 mL，현색시간위70 min。득도제취적우화조건위：을순농도위50%，제취온도위80℃，료액비위1∶20（g/mL），제취시간위120 min。해방법간편，준학，쾌첩。
This research aims at utilizing ultrasonic wave-assisted extraction method to extract the total polyphenol from Loquat nuclear with ethanol-water solution as solvent ,and determining the content of polyphenol by Folin-Ciocalteu Colorimetry.Through the single factor tests and orthogonal experiments ,the optimization of FC conditions and extraction conditions were studied. Finally ,the best colored conditions were as follows:10 %sodium carbonate 1.5 mL, 20%phenol reagent 3.5 mL,colored reaction time 70 min. And the optimum extraction conditions were ethanol concentration 50%, extractive temperature 80℃,material-liquid rate:1∶20 (g/mL), extractive time 120 min.This method is simple ,accurate and fast.
