CAJ | 학술논문

采用微波法和响应面分析法（RSM）对中药紫苏籽中多糖的水提工艺进行了优化。紫苏籽经过粉碎和石油醚脱脂，在单因素实验基础上，选择微波提取功率及提取时间、液料比为自变量，用最陡爬坡路径逼近最大提取率区域后，利用响应面中心组合优化设计了紫苏多糖的最佳水提工艺。结果表明：紫苏多糖的最佳水提工艺为微波功率610 W，微波提取时间1.31 min、液料比28.5∶1，紫苏多糖的提取率为1.99%，并对响应面法优化的提取工艺进行了验证，相差率为0.3%。本结论为紫苏多糖的研究提供了一种方便快捷的提取方法。
채용미파법화향응면분석법（RSM）대중약자소자중다당적수제공예진행료우화。자소자경과분쇄화석유미탈지，재단인소실험기출상，선택미파제취공솔급제취시간、액료비위자변량，용최두파파로경핍근최대제취솔구역후，이용향응면중심조합우화설계료자소다당적최가수제공예。결과표명：자소다당적최가수제공예위미파공솔610 W，미파제취시간1.31 min、액료비28.5∶1，자소다당적제취솔위1.99%，병대향응면법우화적제취공예진행료험증，상차솔위0.3%。본결론위자소다당적연구제공료일충방편쾌첩적제취방법。
In this study, the single factor experiments and the response surface analysis (RSM) were used to optimize the water extraction process of polysaccharide from basil seed, a kind of Chinese medicine. The basil seed was smashed and skimmed using aether petrolei, then the single factor experiments were performed using microwave extraction power and time, material/liquid ratio as independent variables. Finally, the optimal levels of each factor were determined after the peak response area was impended by the steepest climbing method and the central composite response test and were carried out at the apogee.The response surface method results showed that the best extraction process was as follows: microwave power at 610 W, microwave extraction time for 1.31 min, and material/liquid ratio at 28.5∶1,under these conditions, the Perilla polysaccharides extraction rate is 1.99%, and the response surface method to optimize the extraction process was verified with 0.3 % difference. This conclusion was provided a convenient and rapid method for extraction of the polysaccharide.