CAJ | 학술논문

首次通过响应曲面方法优化获得了超声波辅助提取深海红鱼（Sebastes mentella）胶原蛋白的最佳工艺：超声功率505 W，超声时间15 min、提取时间18 h，在此条件下，胶原蛋白提取率（93.6%）与传统提取方法无显著性差异，但提取时间缩短了30 h。该胶原蛋白主要为I型，具有较低的亚氨基酸含量（16.0%），并且由于N末端和C末端非螺旋区的丢失，其分子结构有所改变，但大量氢键的存在使其特有的三股螺旋结构仍占主导地位。
수차통과향응곡면방법우화획득료초성파보조제취심해홍어（Sebastes mentella）효원단백적최가공예：초성공솔505 W，초성시간15 min、제취시간18 h，재차조건하，효원단백제취솔（93.6%）여전통제취방법무현저성차이，단제취시간축단료30 h。해효원단백주요위I형，구유교저적아안기산함량（16.0%），병차유우N말단화C말단비라선구적주실，기분자결구유소개변，단대량경건적존재사기특유적삼고라선결구잉점주도지위。
The process parameters of ultrasonic-assisted extraction of collagen from deep-sea redfish (Sebastes mentella) was optimized using response surface methodology:ultrasonic power 505 W, ultrasonic time 15 min, extraction time 18 h. Under the optimum conditions, the yield of collagen (93.6%) was not significantly different from that prepared by conventional method, but the extraction time was decreased 30 h. The collagen was type I mainly with lower imino acid content (16.0%). The structure was changed slightly due to the loss of N-and C-terminus domains, but the triple helical structure was still predominant as a result of the formation of more hydrogen bond.